Document Detail


Characterization of a glucocorticoid receptor and the direct effect of dexamethasone on herpes simplex virus infection of rabbit corneal cells in culture.
MedLine Citation:
PMID:  7129809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Homogenates prepared from a previously established cell line derived from rabbit cornea contain a macromolecule with many properties of a glucocorticoid receptor, namely high affinity (KD = 6 x 10(-9)M) and saturable capacity (135 femtomoles/mg protein) for dexamethasone, extreme heat lability, and a pattern of competition similar to that found in other glucocorticoid target cells. Intact cells specifically bind dexamethasone with an affinity similar to that found in homogenates, and the amount of steroid bound at saturation is approximately 60,000 molecules of dexamethasone per cell. Specific dexamethasone binding was found to be localized to the cell nucleus. The corneal cells were susceptible to infection with herpes simplex virus (HSV). Dexamethasone increased cell susceptibility to the virus and facilitated the spread of the infection throughout the corneal cell culture. This effect was observed at concentrations of dexamethasone as low as 10(-9) M. Tetrahydrocortisol, an inactive glucocorticoid metabolite that does not compete with dexamethasone binding to the receptor, did not enhance HSV infection at a high concentration (10(-5) M). This study demonstrates a direct effect of dexamethasone on corneal cell-HSV interaction in the absence of exogenous immunologic factors. This effect of dexamethasone may be mediated by the glucocorticoid receptor.
Authors:
B I Weinstein; J Schwartz; G G Gordon; M O Dominguez; S Varma; M W Dunn; A L Southren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  23     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  651-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive
Cells, Cultured
Cornea / cytology,  drug effects*,  physiopathology
Dexamethasone / metabolism,  pharmacology*
Keratitis, Dendritic / metabolism,  physiopathology*
Rabbits
Receptors, Glucocorticoid / metabolism*,  physiology
Receptors, Steroid / metabolism*
Grant Support
ID/Acronym/Agency:
507RR05398/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Glucocorticoid; 0/Receptors, Steroid; 50-02-2/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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