Document Detail


Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives.
MedLine Citation:
PMID:  23046923     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pear-derived Weissella sp. TN610 produced extracellular glycosyltransferase activity responsible for the synthesis of soluble exopolysaccharide from sucrose. Acid and dextranase-catalyzed hydrolysis revealed that the synthesized polymer was a glucan. According to (1)H and (13)C NMR analysis, the glucan produced by TN610 was a linear dextran made of 96% α-(1→6) and 4% α-(1→3) linkages. Zymogram analysis confirmed the presence of a unique glucansucrase of approximately 180kDa in the cell-free supernatant from TN610. The crude enzyme, optimally active at 37°C and pH 5, has promising potential for application as a food additive since it catalyzes dextran synthesis in sucrose-supplemented milk, allowing its solidification. A 4257-bp product corresponding to the mature glucansucrase gene was amplified by PCR from TN610. It encoded a polypeptide of 1418 residues having a calculated molecular mass of 156.089kDa and exhibiting 96 and 95% identity with glucansucrases from Lactobacillus fermentum Kg3 and Weissella cibaria CMU, respectively.
Authors:
Wacim Bejar; Valérie Gabriel; Myriam Amari; Sandrine Morel; Monia Mezghani; Emmanuelle Maguin; Catherine Fontagné-Faucher; Samir Bejar; Hichem Chouayekh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-6
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Université de Toulouse, Laboratoire de Biotechnologies Alimentaires et Environnementales, EA 4565 (LBAE), Institut Universitaire de Technologie, Université Paul Sabatier, 24 rue d'Embaquès, F-32000 Auch, France; Université de Toulouse; INSA, UPS, INP, LISBP, 135 avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; INRA, UMR1319 Micalis, F-78350 Jouy en Josas, France; AgroParisTech, UMR Micalis, F-78350 Jouy en Josas, France.
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