Document Detail


Characterization of the genomic structure and expression of the mouse Apex2 gene.
MedLine Citation:
PMID:  12573260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3' end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5' end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2(+) but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2(+) cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.
Authors:
Yasuhito Ide; Daisuke Tsuchimoto; Yohei Tominaga; Yukihide Iwamoto; Yusaku Nakabeppu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  81     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-02-07     Completed Date:  2003-09-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  47-57     Citation Subset:  IM    
Affiliation:
Division of Neurofunctional Genomics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Fukuoka 812-8582, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB072498;  AB085234;  AB085235
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Chromosome Mapping
DNA Repair / genetics*,  physiology
Endonucleases / genetics,  metabolism
Gene Targeting
Mice
Molecular Sequence Data
Chemical
Reg. No./Substance:
EC 3.1.-/Apex2 protein, mouse; EC 3.1.-/Endonucleases

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