Document Detail

Characterization and gene expression of nodulin Npv30 from common bean.
MedLine Citation:
PMID:  7480336     Owner:  NLM     Status:  MEDLINE    
We previously reported that transcripts for a 30-kD nodulin (Npv30) are very abundant in the nodule. In this paper we describe the isolation and characterization of Npv30 cDNA and genomic clones. Npv30 has the following characteristic features: (a) a putative signal sequence at the deduced amino-terminal region, (b) a proline-rich stretch at the carboxy terminus, and (c) a characteristic domain of four cysteines that resemble metal-binding sites. In Phaseolus vulgaris L., Npv30 is encoded by a small gene family that shares discrete sequence homologies with another small gene family in soybean. An antibody against a beta-galactosidase-Npv30 fusion protein detected two proteins of 28 and 30 kD. Although Npv30 transcripts are very abundant, they encode proteins that are hardly detected in nodule fractions, suggesting that these proteins have a short half-life and/or the mRNAs are strongly regulated at the translational level. Npv30 transcripts were detected in the infected cells of the nodule by in situ hybridization experiments.
F Campos; C Carsolio; H Kuin; T Bisseling; M Rocha-Sosa; F Sánchez
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  109     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-12-18     Completed Date:  1995-12-18     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  363-70     Citation Subset:  IM    
Departamento de Biología Molecular de Plantas, Universidad Nacional Autónoma de México, Cuernavaca.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Z30345;  Z30347
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA, Plant / analysis,  metabolism
Fabaceae / genetics,  metabolism*
Gene Expression*
Genes, Plant
Membrane Proteins / biosynthesis,  chemistry,  genetics
Molecular Sequence Data
Multigene Family
Plant Proteins / biosynthesis*,  chemistry,  genetics
Plants, Medicinal*
RNA, Plant / biosynthesis
Recombinant Fusion Proteins / biosynthesis
Restriction Mapping
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Soybeans / metabolism
Transcription, Genetic
beta-Galactosidase / biosynthesis
Reg. No./Substance:
0/DNA, Plant; 0/Membrane Proteins; 0/NPV30-1 protein, Phaseolus vulgaris; 0/Plant Proteins; 0/RNA, Plant; 0/Recombinant Fusion Proteins; 0/nodulin; EC

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