Document Detail

Characterization of gangliosides from equine kidney and spleen.
MedLine Citation:
PMID:  7451409     Owner:  NLM     Status:  MEDLINE    
Gangliosides were isolated from equine kidney and spleen, and their carbohydrate and lipid moieties were characterized. Among the long-chain bases, considerable proportions of trihydroxy bases (42.3 to 61.2% of the total bases), in which phytosphingosine was predominant were found in all the ganglioside classes. The other major base was sphingosine. Among the constituent fatty acids, long-chain acids (with a carbon number of more than 20), comprised approximately half the total acids, with some alpha-hydroxy and mono-unsaturated acids. By means of sequential hydrolysis with glycosidases coupled with methylation analysis, the following kidney and spleen gangliosides were characterized: hematoside containing both N-acetyl and N-glycolylneuraminic acids; ganglioside GM2 having both types off sialic acid; ganglioside GM1 having mainly N-glycolylneuraminic acid (83 to 86%); ganglioside GD1a having mainly N-glycolylneuraminic acid (71 to 95%). Ganglioside GD3 containing almost exclusively N-glycolylneuraminic acid (93%) was found in the kidney, and ganglioside GD1b having both types of sialic acid in the spleen.
S Gasa; A Makita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  88     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1980 Oct 
Date Detail:
Created Date:  1981-03-17     Completed Date:  1981-03-17     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1119-28     Citation Subset:  IM    
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Ceramides / analysis
Fatty Acids / analysis
Gangliosides / analysis*
Glycosphingolipids / analysis
Kidney / analysis*
Sialic Acids / analysis
Sphingosine / analysis
Spleen / analysis*
Reg. No./Substance:
0/Ceramides; 0/Fatty Acids; 0/Gangliosides; 0/Glycosphingolipids; 0/Sialic Acids; 123-78-4/Sphingosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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