Document Detail


Characterization of gamma-glutamyltransferase from neoplastic and non-neoplastic liver tissues in man and during rat liver hepatocarcinogenesis.
MedLine Citation:
PMID:  2888657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activity and the affinity for concanavalin A-Sepharose (Con A) of liver gamma-glutamyltransferase (gamma GT) were investigated in man, under various clinical conditions and in rats during experimental hepatocarcinogenesis. In man, gamma GT activity was higher than normal in hepatomas and (except for 1 case of hemochromatosis) also higher in the surrounding cirrhotic liver. The proportion of gamma GT which did not bind to Con A (Con A- form) was also increased in the tumors and in the surrounding liver, yet (with the same exception as above) to a greater extent in the hepatomas. In rat, gamma GT activity was higher in fetal liver (15-fold) and in hepatocarcinomas (10-fold) than in normal adult liver; total liver gamma GT activity gradually increased during progression from foci of altered cells to neoplastic nodules and tumors. The proportion of the Con A- form of gamma GT in the early or late stage of the carcinogenic process did not significantly differ from that in normal adult or regenerating rat liver, i.e. about 20% of the total activity. By contrast, nearly all the gamma GT from fetal rat liver bound to Con A. This suggests that gamma GT expression in rat liver carcinoma does not correspond to so-called retrodifferentiation process.
Authors:
M Delhaye; B Gulbis; N Mairesse; P Galand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer & clinical oncology     Volume:  23     ISSN:  0277-5379     ISO Abbreviation:  Eur J Cancer Clin Oncol     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-10-29     Completed Date:  1987-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112045     Medline TA:  Eur J Cancer Clin Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  723-30     Citation Subset:  IM    
Affiliation:
Medicosurgical Department of Gastroenterology, Erasme Hospital, Bruxelles, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Animals
Carcinoma, Hepatocellular / enzymology*
Chromatography, Affinity
Concanavalin A
Female
Humans
Isoenzymes / analysis*
Liver / embryology,  enzymology*
Liver Neoplasms / enzymology*
Liver Neoplasms, Experimental / enzymology
Liver Regeneration
Male
Middle Aged
Rats
Rats, Inbred Strains
gamma-Glutamyltransferase / analysis*
Chemical
Reg. No./Substance:
0/Isoenzymes; 11028-71-0/Concanavalin A; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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