Document Detail


Characterization of gamma-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao.
MedLine Citation:
PMID:  17090946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gamma-glutamyl hydrolase with a molecular mass of 28 kDa was purified from the culture broth of Bacillus sp. isolated from Thai Thua-nao, a natto-like fermented soybean food. The purified enzyme hydrolyzed chemically synthesized oligo-gamma-L-glutamates but not oligo-gamma-D-glutamates and degraded gamma-polyglutamic acid to a hydrolyzed product of only about 20 kDa (with D- and L-glutamic acid in a ratio of 70:30), suggesting that the enzyme is a gamma-glutamyl hydrolase that cleaves the gamma-glutamyl linkage between L- and L-glutamic acid of gamma-polyglutamic acid.
Authors:
Orawan Chunhachart; Takenori Itoh; Morakot Sukchotiratana; Hiroyuki Tanimoto; Yasutaka Tahara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-24     Completed Date:  2007-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2779-82     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bacillus / enzymology*,  isolation & purification*
Chromatography, High Pressure Liquid
Fermentation
Hydrolysis
Polyglutamic Acid / metabolism
Soybeans / microbiology*
Thailand
gamma-Glutamyl Hydrolase / metabolism*
Chemical
Reg. No./Substance:
25513-46-6/Polyglutamic Acid; EC 3.4.19.9/gamma-Glutamyl Hydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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