Document Detail


Characterization of a fucoarabinogalactan, the main polysaccharide from the gum exudate of Croton urucurana.
MedLine Citation:
PMID:  12193018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fucoarabinogalactan (CU-1), the main component of the gum exudate of the medicinal plant Croton urucurana, has been isolated by precipitation and subsequent dialysis and finally purified by ion-exchange chromatography. The estimated average molecular weight of CU-1 by gel permeation chromatography was approximately 2.48 x 10(6) Da. CU-1 was found to contain 1.0% proteins and 93.7% total sugars, mainly fucose, arabinose, and galactose (molar ratio: 7.8, 8.1, 19.0), and minor quantities of mannose, xylose, glucose, and uronic acids (molar ratio: 2.2, 1.0, 0.3, 3.0). Among the uronic acids, glucuronic acid was identified and the presence of mannuronic acid could be also presumed. Methylation analysis of this polysaccharide revealed high proportions of 1,3-linked, 1,2,3-linked, and 1,2,3,6-linked galactose, 1-linked fucose, and 1-linked arabinose. This suggests that CU-1 is constituted by a principal skeleton of (1-->3)-linked galactopyranose units, with some of these galactose units branched at the 2-position or at both the 2- and 6-positions, and with mainly terminal fucopyranosyl and arabinofuranosyl residues in the side chains. This is the first report on the polysaccharide constitution of a gum exudate from a Croton species.
Authors:
Begoña Milo; Ester Risco; Roser Vila; José Iglesias; Salvador Cañigueral
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of natural products     Volume:  65     ISSN:  0163-3864     ISO Abbreviation:  J. Nat. Prod.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-23     Completed Date:  2002-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7906882     Medline TA:  J Nat Prod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1143-6     Citation Subset:  IM    
Affiliation:
Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Avinguda Diagonal, 643, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Croton / chemistry*
Galactans / chemistry*,  isolation & purification*
Gas Chromatography-Mass Spectrometry
Hydrolysis
Methylation
Paraguay
Plants, Medicinal / chemistry*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Galactans; 0/fucoarabinogalactan

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