Document Detail


Characterization of free surface microprojections on the kidney glomerular epithelium.
MedLine Citation:
PMID:  7024999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Scanning and transmission electron microscopy were used to evaluate free surface microprojections on the kidney glomerular epithelium in response to various experimental situations. Normally glomerular epithelial podocytes exhibit a rather sparse population of finger-like or bleb-like projections. Some investigations, however, have suggested that microvillous projections may increase in number during the differentiation of podocytes and in the aging kidney. With the onset of puromycin aminonucleoside induced nephrotic syndrome, podocyte microprojections become very knob-like and irregular in their shapes. In response to the in vitro environment, the number of podocyte microprojections increases dramatically. Neuraminidase removal of the sialic acid component of the glomerular glycocalyx and exposure to either cytochalasin B (25 microgram/ml) or cytochalasin D (2 microgram/ml), prevent the in vitro formation of podocyte microprojections. In vitro incubation in compounds which depolymerize cytoplasmic microtubules (e.g. vinblastine sulfate 10(-5); colchicine 10(-5) M), however, result in the formation of unusually long glomerular epithelial microprojections. Investigations utilizing the cationic ligand polycationized ferritin (PCF) have shown that podocyte microprojections are the most anionic sites on the free surfaces of these cells. During prolonged incubation of PCF treated glomeruli, the tips of podocyte microprojections are the last sites to shed the anionically bound PCF. It is suggested that the highly anionic sialic acid components of the glomerular free surface glycocalyx may play principal roles in the formation, maintenance and shapes of glomerular podocyte microprojections.
Authors:
P M Andrews
Related Documents :
8527849 - Lectin binding and identification of sialic acid acceptor sugars in rabbit oviduct unde...
10334849 - Core-branching pattern and sequence analysis of mannitol-terminating oligosaccharides b...
7085639 - Purification and macromolecular properties of a sialic acid-specific lectin from the sl...
2249689 - A study on the regulation of n-glycoloylneuraminic acid biosynthesis and utilization in...
9192099 - Further biochemical and biophysical characterisation of scyllin, scylla serrata hemolym...
4853069 - Major polar lipids of hepatitis b antigen preparations: evidence for the presence of a ...
9742699 - Pkc1, encoding a protein kinase c, and fat1, encoding a fatty acid transporter protein,...
24931969 - Yolk sac fatty acid composition, yolk absorption, embryo development, and chick quality...
1840619 - Molecular basis of viral persistence: a single amino acid change in the glycoprotein of...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Progress in clinical and biological research     Volume:  59B     ISSN:  0361-7742     ISO Abbreviation:  Prog. Clin. Biol. Res.     Publication Date:  1981  
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7605701     Medline TA:  Prog Clin Biol Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  21-35     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / ultrastructure*
Cytochalasin B / pharmacology
Cytochalasin D
Cytochalasins / pharmacology
Epithelium / ultrastructure
Ferritins / metabolism
Kidney Glomerulus / drug effects,  ultrastructure*
Microscopy, Electron, Scanning
Microtubules / physiology
Microvilli / ultrastructure*
Nephrotic Syndrome / pathology
Neuraminidase / pharmacology
Polymers / metabolism
Rats
Grant Support
ID/Acronym/Agency:
AM25417/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Cytochalasins; 0/Polymers; 0/polycationic ferritin; 14930-96-2/Cytochalasin B; 22144-77-0/Cytochalasin D; 9007-73-2/Ferritins; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Three-dimensional microanatomy of intracellular structures.
Next Document:  Morphophysiologic bases for the predisposition of the bovine lung to bronchial pneumonia.