Document Detail


Characterization of food spoilage fungi by FTIR spectroscopy.
MedLine Citation:
PMID:  23210658     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: The objective of the study was to evaluate a high-throughput liquid microcultivation protocol and FTIR spectroscopy for differentiation of food spoilage filamentous fungi. METHODS AND RESULTS: For the study, fifty nine food-related fungal strains were analyzed. The cultivation of fungi was performed in liquid medium in the Bioscreen C micro-titer plate system with a throughput of 200 samples per cultivation run. Mycelium was prepared for FTIR analysis by a simple procedure, including a washing and a homogenization step. Hierarchical cluster analysis was used to study affinity among the different species. Based on the hierarchical cluster analysis, a classification and validation scheme was developed by artificial neural network analysis. The classification network was tested by an independent test set. The results show that 93.9% and 94.0% of the spectra were correctly identified at the species and genus level respectively. CONCLUSIONS: The use of high-throughput liquid microcultivation protocol combined with FTIR spectroscopy and artificial neural network analysis allows differentiating of food spoilage fungi on the phylum, genus and species level. © 2012 © 2012 Nofima AS Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
V Shapaval; J Schmitt; T Møretrø; H P Suso; I Skaar; A Wold Åsli; D Lillehaug; A Kohler
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 © 2012 Nofima AS Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Nofima AS, Osloveien 1, N-1430 Ås, Norway; Centre for Integrative Genetics (CIGENE), Department of Mathematical Sciences and Technology, Norwegian University of Life Sciences, Postbox 5003, 1432 Ås, Norway.
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