| Characterization of folate-dependent enzymes and indices of folate status in laying hens supplemented with folic acid or 5-methyltetrahydrofolate. | |
| | |
MedLine Citation:
|
PMID: 20308400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The conversion of folic acid (FA) to the biologically active 5-methyltetrahydrofolate (5-MTHF) is necessary for the deposition of folate in the egg. A study was conducted to compare egg folate concentrations, indices of folate status, and activities of folate-dependent enzymes in response to equimolar intake of either FA or 5-MTHF in laying hens. Forty-eight laying hens, 24 wk of age, from 2 different strains (Shaver White and Shaver Brown) were randomly assigned to receive 1 of 3 (n = 8 per strain) dietary treatments: 1) basal diet with no supplemental folate, 2) basal diet + 10 mg/kg of FA, or 3) basal diet + 11.3 mg/kg of 5-MTHF for 3 wk. A completely randomized design with 3 dietary treatments and 2 laying hen strains in a 3 x 2 factorial arrangement was used. Data were subjected to ANOVA, using the PROC GLM procedure of SAS. Plasma homocysteine, serum, and egg folate concentrations; hepatic serine hydroxymethyltransferase; and methionine synthase activity were affected by dietary folate supplementation but not by its form (FA and 5-MTHF). Relative to control hens, plasma homocysteine was decreased (P < 0.05) by 14.2%, whereas serum and egg folate were increased (P < 0.05) by 78.3 and 61.8%, respectively, in hens consuming either folate compound. Hepatic serine hydroxymethyltransferase and methionine synthase activity were increased and decreased (P < 0.05), respectively, in folate-fed birds compared with control-fed birds. Hepatic dihydrofolate reductase was influenced by both the addition and form of dietary folate, being higher (P < 0.05) in FA-fed birds than in 5-MTHF and control-fed birds. Feed efficiency was improved (P < 0.05) in 5-MTHF-fed birds relative to FA-fed birds. Strain of hen influenced serum folate and plasma homocysteine concentrations but not other indices of folate metabolism. Overall, FA and 5-MTHF have equivalent effects in enhancing egg folate concentrations and improving folate status in laying hens. Also, supplementation and form of folate may modulate the activity of folate-dependent enzymes. |
| | |
Authors:
|
G B Tactacan; M Jing; S Thiessen; J C Rodriguez-Lecompte; D L O'Connor; W Guenter; J D House |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Poultry science Volume: 89 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2010 Apr |
Date Detail:
|
Created Date: 2010-03-23 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 688-96 Citation Subset: IM |
Affiliation:
|
Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Feed Animals Chickens Dietary Supplements Duodenum / enzymology Energy Intake Female Folic Acid / metabolism*, pharmacology* Glycine Hydroxymethyltransferase / metabolism* Liver / enzymology Methylenetetrahydrofolate Reductase (NADPH2) / metabolism* Oviposition Tetrahydrofolate Dehydrogenase / metabolism* Tetrahydrofolates / metabolism*, pharmacology* Triticum |
| Chemical | |
Reg. No./Substance:
|
0/Tetrahydrofolates; 134-35-0/5-methyltetrahydrofolate; 59-30-3/Folic Acid; EC 1.5.1.20/Methylenetetrahydrofolate Reductase (NADPH2); EC 1.5.1.3/Tetrahydrofolate Dehydrogenase; EC 2.1.2.1/Glycine Hydroxymethyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of the duration of prefeeding on amino acid digestibility of wheat distillers dried grains wi...
Next Document: Regulation of chick bone growth by leptin and catecholamines.