Document Detail


Characterization of five chemistries and three particle sizes of stationary phases used in supercritical fluid chromatography.
MedLine Citation:
PMID:  24377105     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Sub-2-microns particles employed as supporting phases are known to favor column efficiency. Recently a set of columns based on sub-2-microns particles for use with supercritical fluid mobile phases have been introduced by Waters. Five different stationary phase chemistries are available: BEH silica, BEHEthyl-pyridine, X Select CSH Fluorophenyl, HSS C18 SB and BEH Shield RP18. This paper describes the characterization of 15 stationary phases, the five different chemistries, and three particle sizes, 1.7 (or 1.8), 3.5 and 5 microns, with the same carbon dioxide–methanol mobile phase and a set of more than a hundred compounds. The interactions established in the 15 different chromatographic systems used in supercritical fluid chromatography (SFC) are assessed with linear solvation energy relationships (LSERs).The results show the good complementarity of the five column chemistries, and their comparative location inside a classification map containing today around 70 different commercial phases. Among the five different chemistries, the HSS C18 SB phase displays a rather unusual behavior in regards of classical C18 phases, as it displays significant hydrogen–bonding interactions. Besides, it appears, as expected, that the BEH Ethyl–pyridine phase has weak interactions with basic compounds. The effect of particle size was studied because smaller particles induce increased inlet and internal pressure. For compressible fluids,this pressure change modifies the fluid density, i.e. the apparent void volume and the eluting strength.These changes could modify the retention and the selectivity of compounds in the case of method trans-fer, by using different particle sizes, from 5 down to 1.7 m. A hierarchical cluster analysis shows that stationary phase clusters were based on the phase chemistry rather than on the particle size, meaning that method transfer from 5 to 1.7 microns can be achieved in the subcritical domain i.e. by using a weakly compressible fluid.
Authors:
S Khater; C West; E Lesellier
Related Documents :
24517295 - Chamber studies on non-vented decorative fireplaces using liquid or gelled ethanol fuel.
20576475 - Predictable and linear scale-up of four phenolic alkaloids separation from the roots of...
23607505 - Reliable peak selection for multisample analysis with comprehensive two-dimensional chr...
15546765 - Determination of moisture fraction in wood by mobile nmr device.
621655 - Rapid high-pressure liquid chromatographic determination of quinidine and dihydroquinid...
24731775 - Physiology of geobacter metallireducens under excess and limitation of electron donors....
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1319     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  148-59     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An integrated strategy of ultra-high-performance liquid chromatography/quadrupole-time-of-flight mas...
Next Document:  Spatial and spatio-temporal models with R-INLA.