Document Detail

Characterization of fibromodulin isolated from bovine periodontal ligament.
MedLine Citation:
PMID:  9524315     Owner:  NLM     Status:  MEDLINE    
Although several proteoglycans (PGs) have been reported in bovine periodontal ligament (PDL), the composition of PGs in PDL has been poorly characterized. In the present study, we isolated and characterized keratan sulfate-substituted PG (fibromodulin) in bovine PDL. Fibromodulin was purified from 4 M guanidine hydrochloride (GdmCl) extracts of bovine PDL tissues using DEAE Sephacel ion-exchange chromatography and preparative electrophoresis. Fibromodulin appeared as a single polydisperse band with an apparent molecular weight (MW) of 80,000 (80 kDa) on SDS-PAGE. Digestion of fibromodulin with keratanase or neuraminidase reduced the apparent molecular size, and N-glycanase treatment produced core protein bands of around 40 kDa. Fibromodulin reacted with keratan sulfate monoclonal antibody (5D4) and fibromodulin polyclonal antibodies (alpha-FM). The keratanase-digested fibromodulin reacted with alpha-FM, but not with 5D4. These data suggest that fibromodulin is one of the small PGs in the PDL-matrix and may fulfill construction and maintenance functions in this tissue.
T Watanabe; T Kubota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of periodontal research     Volume:  33     ISSN:  0022-3484     ISO Abbreviation:  J. Periodont. Res.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-05-14     Completed Date:  1998-05-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0055107     Medline TA:  J Periodontal Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  D; IM    
Department of Orthodontics, Kanagawa Dental College, Yokosuka, Japan.
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MeSH Terms
Amidohydrolases / chemistry
Antibodies, Monoclonal / diagnostic use
Carrier Proteins / analysis*,  chemistry,  immunology,  isolation & purification
Chromatography, Agarose
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix Proteins*
Glycoside Hydrolases*
Guanidine / chemistry
Keratan Sulfate / analysis,  immunology
Molecular Structure
Molecular Weight
Neuraminidase / chemistry
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Periodontal Ligament / chemistry*
Proteoglycans / analysis
Sodium Dodecyl Sulfate
beta-Galactosidase / chemistry
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Carrier Proteins; 0/Extracellular Matrix Proteins; 0/Proteoglycans; 113-00-8/Guanidine; 126468-95-9/fibromodulin; 151-21-3/Sodium Dodecyl Sulfate; 9056-36-4/Keratan Sulfate; EC 3.2.1.-/Glycoside Hydrolases; EC endo-1,4-beta-galactosidase; EC; EC; EC 3.5.-/Amidohydrolases; EC Asparagine Amidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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