| Characterization of fetal haematopoietic progenitors circulating in maternal blood of seven aneuploid pregnancies. | |
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MedLine Citation:
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PMID: 9467814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A retrospective study was carried out in order to investigate the phenotype of fetal haematopoietic progenitors circulating in the maternal blood of seven aneuploid pregnancies. Five of the blood samples were taken during pregnancies affected by various fetal aneuploidies, while the other two were collected after therapeutic abortion due to prenatal cytogenetic diagnosis of trisomies 21 and 18. Haematopoietic progenitor cells, isolated by labelling the erythropoietin receptors with the biotinylated ligand before magnetic sorting and/or fibronectin cell adhesion assay, were cultured in a suitable semisolid medium. Single- or dual-colour fluorescence in situ hybridization (FISH) was utilized to identify and enumerate fetal cells amplified in culture. Fetal trisomies were confirmed in the FISH analysis with chromosome-specific probes in all the cases analysed. The fetal purity rate ranged from 16 to 26 per cent. Haematopoietic progenitors of fetal origin were found to include CFU-E, CFU-GEMM, and possibly also M-BFU-E. Interestingly, a more immature progenitor with high self-renewal capacity (CFU-blast cell) isolated by fibronectin sorting was shown to have a relatively high frequency in one case of Down syndrome. In general, the results of this study demonstrate the feasibility of diagnosing the major fetal chromosomopathies by culturing fetal cells taken from maternal blood. Furthermore, our initial data on the sequential sorting for fibronectin and erythropoietin receptors lead us to believe that this approach may broaden the range of fetal haematopoietic progenitors retrievable from the maternal circulation. |
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Authors:
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D Valerio; V Altieri; F R Antonucci; R Aiello |
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Publication Detail:
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Type: Case Reports; Comparative Study; Journal Article |
Journal Detail:
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Title: Prenatal diagnosis Volume: 17 ISSN: 0197-3851 ISO Abbreviation: Prenat. Diagn. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-03-12 Completed Date: 1998-03-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1159-69 Citation Subset: IM |
Affiliation:
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Istituto di Ricerche Genetiche, Napoli, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aneuploidy* Cells, Cultured Chromosomes, Human, Pair 13* Chromosomes, Human, Pair 18* Down Syndrome / diagnosis*, embryology, genetics Female Fetal Diseases / diagnosis*, embryology, genetics Gestational Age Hematopoietic Stem Cells / chemistry, cytology* Humans In Situ Hybridization, Fluorescence Karyotyping Phenotype Pregnancy / blood Prenatal Diagnosis / methods* Retrospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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