| Characterization of ferrioxamine E as the principal siderophore of Erwinia herbicola (Enterobacter agglomerans). | |
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MedLine Citation:
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PMID: 2978958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several strains of the enterobacterial group Erwinia herbicola (Enterobacter agglomerans) were screened for siderophore production. After 3 days of growth in a low-iron medium, all strains studied produced hydroxamate siderophores. The retention values of the main siderophore during thin-layer chromatography on silica gel plates and on HPLC reversed-phase columns were identical with those of an authentic sample of ferrioxamine E (norcardamine). Gas-chromatographic analysis of the HI hydrolyzate yielded succinic acid and 1,5-diaminopentane in equimolar amounts; fast-atom-bombardment (FAB) mass spectroscopy showed a molecular mass of 653 Da. Iron from 55Fe-labelled ferrioxamine E was well taken up by iron-starved cells of E. herbicola (Km = 0.1 microM, Vmax = 8 pmol mg-1 min-1). However, besides ferrioxamine E (100%), several exogenous siderophores such as enterobactin (94.5%), ferric citrate (78.5%), coprogen (63.5%) and ferrichrome (17.5%) served as siderophores, suggesting the presence of multiple siderophore receptors in the outer membrane of E. herbicola. |
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Authors:
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I Berner; S Konetschny-Rapp; G Jung; G Winkelmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of metals Volume: 1 ISSN: 0933-5854 ISO Abbreviation: Biol Met Publication Date: 1988 |
Date Detail:
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Created Date: 1990-11-01 Completed Date: 1990-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8915662 Medline TA: Biol Met Country: GERMANY |
Other Details:
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Languages: eng Pagination: 51-6 Citation Subset: IM |
Affiliation:
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Institut für Biologie I, Universität Tübingen, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport, Active Chromatography, High Pressure Liquid Erwinia / metabolism* Ferric Compounds / isolation & purification, metabolism* Iron / metabolism Iron Chelating Agents / isolation & purification, metabolism* Kinetics Mass Spectrometry Molecular Structure Peptides, Cyclic / isolation & purification, metabolism* Siderophores |
| Chemical | |
Reg. No./Substance:
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0/Ferric Compounds; 0/Iron Chelating Agents; 0/Peptides, Cyclic; 0/Siderophores; 20008-20-2/ferrioxamine E; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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