Document Detail

Characterization of the feeding inhibition and neural activation produced by dorsomedial hypothalamic cholecystokinin administration.
MedLine Citation:
PMID:  18248910     Owner:  NLM     Status:  MEDLINE    
Within the dorsomedial hypothalamus (DMH), cholecystokinin (CCK) has been proposed to modulate neuropeptide Y (NPY) signaling to affect food intake. However, the neural circuitry underlying the actions of this CCK-NPY signaling system in the controls of food intake has yet to be determined. We sought to characterize the feeding inhibition and brain neural activation produced by CCK administration into the DMH of rats. We determined the time course of feeding inhibitory effects of exogenous DMH CCK, assessed NPY gene expression in the DMH in response to DMH CCK administration, and characterized c-Fos activation in the entire brain induced by CCK injection into the DMH using c-Fos like immunohistochemistry. We found that parenchymal injection of CCK into the DMH decreased food intake during the entire 22 h observation period, with a primary effect in the first 4 h, and down-regulated NPY gene expression in the DMH. c-Fos immunohistochemistry revealed that DMH CCK increased the number of c-Fos positive cells in the paraventricular nucleus (PVN), arcuate nucleus, suprachiasmatic nucleus and retrochiasmatic area as well as in the contralateral DMH. This pattern of activity is different from that produced by peripherally administered CCK which is short acting and primarily activates neurons in the nucleus of the solitary tract and area postrema, as well as the PVN and DMH. Together, these data suggest that DMH CCK plays an important role in the control of food intake, and does so by activating different pathways from those activated by peripheral CCK.
J Chen; K A Scott; Z Zhao; T H Moran; S Bi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Neuroscience     Volume:  152     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-06-30     Completed Date:  2008-08-05     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  178-88     Citation Subset:  IM    
Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 618, Baltimore, MD 21205, USA.
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MeSH Terms
Cholecystokinin / metabolism*
Eating / physiology*
Gene Expression
Hypothalamus / physiology*
In Situ Hybridization
Neurons / metabolism
Neuropeptide Y / biosynthesis
Proto-Oncogene Proteins c-fos / biosynthesis
Rats, Sprague-Dawley
Grant Support
DK 074269/DK/NIDDK NIH HHS; R01 DK074269-01A1/DK/NIDDK NIH HHS; R01 DK074269-02/DK/NIDDK NIH HHS
Reg. No./Substance:
0/Neuropeptide Y; 0/Proto-Oncogene Proteins c-fos; 9011-97-6/Cholecystokinin

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