| Characterization of extracellular GABA in the substantia nigra reticulata by means of brain microdialysis. | |
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MedLine Citation:
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PMID: 1635589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain microdialysis was used to characterize extracellular gamma-aminobutyric acid (GABA) in the substantia nigra reticulata (SNR) of freely moving rats. The extracellular GABA in the SNR was characterized using acutely implanted probes (4-8 h after surgery; day 1) and chronically implanted probes (24 h after surgery; day 2). 3-Mercaptopropionic acid, a glutamic acid decarboxylase inhibitor, was used to identify GABA. This drug induced an immediate decrease in the extracellular GABA levels to 40% of basal values, suggesting that the detected GABA is, at least in part, newly synthesized. The basal levels of extracellular GABA measured either on day 1 or day 2 were not affected by infusion of micromolar amounts of tetrodotoxin. Therefore, a direct coupling between GABA dialysate concentrations and nerve-impulse flow does not seem to exist. Infusion of the GABA uptake inhibitor nipecotic acid (0.5 mmol/l) resulted in a 4-fold increase in the dialysate levels of GABA lasting at least for 3 h on both days. K+ stimulation (60 mmol/l) increased extracellular GABA levels in the SNR to 450% of basal values. This effect again did not differ significantly on day 1 and day 2. The origin of the extracellular GABA in the SNR, as recorded by microdialysis under the two experimental conditions, is discussed. |
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Authors:
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W Timmerman; J Zwaveling; B H Westerink |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Naunyn-Schmiedeberg's archives of pharmacology Volume: 345 ISSN: 0028-1298 ISO Abbreviation: Naunyn Schmiedebergs Arch. Pharmacol. Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-08-24 Completed Date: 1992-08-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0326264 Medline TA: Naunyn Schmiedebergs Arch Pharmacol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 661-5 Citation Subset: IM |
Affiliation:
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Department of Medicinal Chemistry, University Centre for Pharmacy, Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Mercaptopropionic Acid
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pharmacology Animals Dialysis Extracellular Space / drug effects, metabolism Male Potassium / pharmacology Rats Rats, Inbred Strains Substantia Nigra / drug effects, metabolism* Tetrodotoxin / pharmacology gamma-Aminobutyric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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107-96-0/3-Mercaptopropionic Acid; 4368-28-9/Tetrodotoxin; 56-12-2/gamma-Aminobutyric Acid; 7440-09-7/Potassium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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