Document Detail

Characterization of enzymes involved in formation of ethyl esters of long-chain fatty acids in humans.
MedLine Citation:
PMID:  11441128     Owner:  NLM     Status:  MEDLINE    
Elevated fatty acid ethyl ester (FAEE) concentrations have been detected in postmortem organs from alcoholics and patients acutely intoxicated by alcohol, and FAEE have been implicated as mediators of ethanol-induced organ damage. The formation of FAEE is catalyzed by acyl-coenzyme A:ethanol O-acyltransferase (AEAT) and by FAEE synthase, which utilize acyl-CoA and free fatty acids, respectively, as substrates. Because little is known about the capacity of various human tissues to synthesize and hydrolyze FAEE, we investigated formation of FAEE by AEAT and FAEE synthase in tissue homogenates from human gastric ventricular and duodenal mucosa, pancreas, liver, heart, lung, and adipose tissue, gallbladder mucosa, and in serum. Liver, duodenal mucosa, and pancreas were found to have the highest capacities to synthesize FAEE, mainly due to AEAT. FAEE hydrolyzing activity was highest in liver and pancreas, but hardly detectable in adipose tissue or heart. Because fatty acids and alcohol are absorbed by the intestinal mucosa, intestine may be a major site of FAEE synthesis, and FAEE may be delivered via the circulation to other organs and taken up by lipoprotein receptor-mediated uptake. A very low rate of FAEE hydrolysis was detected in heart and adipose tissue, which probably accounts for the previously observed accumulation of FAEE in these organs.
M A Diczfalusy; I Björkhem; C Einarsson; C G Hillebrant; S E Alexson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  42     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-06     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1025-32     Citation Subset:  IM    
Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, SE-141 86 Stockholm, Sweden.
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MeSH Terms
Acyltransferases / metabolism*
Carboxylic Ester Hydrolases / metabolism*
Duodenum / metabolism
Ethanol / metabolism*
Fatty Acids / metabolism
Microsomes, Liver / metabolism*
Mucous Membrane / metabolism
Pancreas / metabolism
Rats, Sprague-Dawley
Reg. No./Substance:
0/Fatty Acids; 64-17-5/Ethanol; EC 2.3.-/Acyltransferases; EC 2.3.1.-/ethanol acyltransferase; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC; EC acyl ethyl ester synthase

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