Document Detail


Characterization of enteric functional changes evoked by in vivo anti-CD3 T cell activation.
MedLine Citation:
PMID:  10070131     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specific in vivo T cell activation initiated by treatment with anti-CD3 antibodies leads to diarrhea and structural damage of the intestinal mucosa. In this study, the effect of T cell-induced mucosal damage on jejunal epithelial ion transport, muscle contractility, and neuronal ACh release was assessed in Ussing chambers, organ baths, and a specialized perfusion apparatus, respectively. Time-matched control mice received hamster serum containing irrelevant antibodies. Jejunal segments from anti-CD3-treated mice displayed a significantly elevated epithelial baseline short-circuit current (which indicates increased ion transport) and a concomitant reduction in responsiveness to prosecretory stimuli (nerve stimulation, carbachol, and forskolin). Longitudinal smooth muscle displayed altered spontaneous contractile activity, length-tension relationships, and carbachol-stimulated contraction in tissues excised from mice 20 and 40 h posttreatment. Anti-CD3 treatment did not affect stimulated ACh release from myenteric plexus neurons. We conclude that specific T cell activation via anti-CD3 antibody results in dramatic alterations in jejunal epithelial and smooth muscle function. Such T cell-induced changes in intestinal function may contribute to the symptomatology of T cell-mediated enteropathies, including graft-versus-host disease, celiac disease, and idiopathic inflammatory bowel disease.
Authors:
N Radojevic; D M McKay; M Merger; B A Vallance; S M Collins; K Croitoru
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-15     Completed Date:  1999-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R715-23     Citation Subset:  IM    
Affiliation:
Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism
Animals
Antibodies / pharmacology*
Antigens, CD3 / immunology*
Bromodeoxyuridine / metabolism
Cricetinae
Female
Intestinal Mucosa / drug effects,  innervation,  pathology,  physiopathology
Jejunum / drug effects*,  innervation,  pathology,  physiopathology*
Lymphocyte Activation / physiology*
Lymphocytes / metabolism
Mice
Mice, Inbred BALB C
Muscle, Smooth / physiopathology
Neurons / metabolism
T-Lymphocytes / drug effects*,  physiology*
Tumor Necrosis Factor-alpha / biosynthesis
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD3; 0/Tumor Necrosis Factor-alpha; 51-84-3/Acetylcholine; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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