| Characterization of enteric functional changes evoked by in vivo anti-CD3 T cell activation. | |
MedLine Citation:
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PMID: 10070131 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Specific in vivo T cell activation initiated by treatment with anti-CD3 antibodies leads to diarrhea and structural damage of the intestinal mucosa. In this study, the effect of T cell-induced mucosal damage on jejunal epithelial ion transport, muscle contractility, and neuronal ACh release was assessed in Ussing chambers, organ baths, and a specialized perfusion apparatus, respectively. Time-matched control mice received hamster serum containing irrelevant antibodies. Jejunal segments from anti-CD3-treated mice displayed a significantly elevated epithelial baseline short-circuit current (which indicates increased ion transport) and a concomitant reduction in responsiveness to prosecretory stimuli (nerve stimulation, carbachol, and forskolin). Longitudinal smooth muscle displayed altered spontaneous contractile activity, length-tension relationships, and carbachol-stimulated contraction in tissues excised from mice 20 and 40 h posttreatment. Anti-CD3 treatment did not affect stimulated ACh release from myenteric plexus neurons. We conclude that specific T cell activation via anti-CD3 antibody results in dramatic alterations in jejunal epithelial and smooth muscle function. Such T cell-induced changes in intestinal function may contribute to the symptomatology of T cell-mediated enteropathies, including graft-versus-host disease, celiac disease, and idiopathic inflammatory bowel disease. |
Authors:
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N Radojevic; D M McKay; M Merger; B A Vallance; S M Collins; K Croitoru |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 276 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-04-15 Completed Date: 1999-04-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R715-23 Citation Subset: IM |
Affiliation:
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Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario, Canada L8N 3Z5. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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metabolism Animals Antibodies / pharmacology* Antigens, CD3 / immunology* Bromodeoxyuridine / metabolism Cricetinae Female Intestinal Mucosa / drug effects, innervation, pathology, physiopathology Jejunum / drug effects*, innervation, pathology, physiopathology* Lymphocyte Activation / physiology* Lymphocytes / metabolism Mice Mice, Inbred BALB C Muscle, Smooth / physiopathology Neurons / metabolism T-Lymphocytes / drug effects*, physiology* Tumor Necrosis Factor-alpha / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigens, CD3; 0/Tumor Necrosis Factor-alpha; 51-84-3/Acetylcholine; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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