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Characterization of an enhancer upstream from the muscle-type promoter of a gene encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
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MedLine Citation:
PMID:  1322527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The muscle-type isozyme of rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is encoded by a mRNA transcribed from the M promoter of a 55-kb gene, which also produces the liver-type isozyme from an alternative promoter. By transient transfection and in vitro protein-DNA binding assays we have delineated, within 4.7 kb of 5' flanking sequence, the M promoter proper and an enhancer located between -1615 and -1809. This enhancer stimulated up to 12-fold the activity of the promoter in the context of an intact 5' flanking sequence and close to 900-fold the activity of the minimal (+41 to -40) M promoter cloned directly downstream from it. A functional dissection of the enhancer by site-directed mutagenesis and use of oligonucleotides suggested that its activity involves the cooperative effect of six binding sites for trans-acting factors clustered within 150 bp. These sites contain either an EF-1A/E4TF1 motif (also known to bind the ets oncogene product) or a Sp1 motif, or both. The activity of the enhancer could be demonstrated in L6 myoblasts and myocytes and in FTO2B hepatoma cells. When left within the intact 5' flanking sequence, however, enhancer activity was inhibited upon differentiation of myoblasts into myocytes.
Authors:
M I Darville; I V Antoine; G G Rousseau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  20     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-09-02     Completed Date:  1992-09-02     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3575-83     Citation Subset:  IM    
Affiliation:
Hormone and Metabolic Research Unit, Louvain University Medical School, Brussels, Belgium.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M26215
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites / genetics
Enhancer Elements, Genetic / genetics*,  physiology
Molecular Sequence Data
Muscles / enzymology*
Mutagenesis, Site-Directed / genetics
Oligodeoxyribonucleotides / genetics
Phosphofructokinase-2
Phosphoric Monoester Hydrolases / genetics*
Phosphotransferases / genetics*
Plasmids / genetics
Promoter Regions, Genetic / genetics
Rats
Transcription Factors / metabolism
Transfection / genetics
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides; 0/Transcription Factors; EC 2.7.-/Phosphotransferases; EC 2.7.1.105/Phosphofructokinase-2; EC 3.1.3.-/Phosphoric Monoester Hydrolases
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 7 Year: 1992
Volume: 20 Issue: 14
First Page: 3575 Last Page: 3583
ID: 334004
PubMed Id: 1322527

Characterization of an enhancer upstream from the muscle-type promoter of a gene encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
M I Darville
I V Antoine
G G Rousseau
Hormone and Metabolic Research Unit, Louvain University Medical School, Brussels, Belgium.



Article Categories:
  • Research Article


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