| Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic. | |
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MedLine Citation:
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PMID: 22524528 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum , Rhizoctonia solani , Colletotrichum gloeosporioides , Cryphonectria parasitica , Fusarium oxysporum , Pyrenophora tritici-repentis , Epidermophyton floccosum , and Trichophyton rubrum , was isolated from surface-sterilized Catharanthus roseus stems. Preliminary identification showed that Streptomyces cinnamoneus subsp. sparsus was its closest related species. However, strain TS3RO could readily be distinguished from this species using a combination of phenotypic properties, 16S rDNA sequence similarity, and phylogenetic analyses. Thus, the whorl-forming Streptomyces sp. strain TS3RO is likely a new subspecies within the Streptomyces cinnamoneus group. Direct bioautography on a thin-layer chromatography plate with Cladosporium cucumerinum was conducted throughout the purification steps for bioassay-guided isolation of the active antifungal compounds from the crude extract. Structural elucidation of the isolated bioactive compound was obtained via LC-MS spectrometry, UV-visible spectra, and nuclear magnetic resonance data. It revealed that fungichromin, a known methylpentaene macrolide antibiotic, was the main antifungal component of TS3RO strain, as shown by thin-layer chromatography bioautography. This is the first report of an endophytic whorl-forming Streptomyces isolated from the medically important plant Catharanthus roseus. |
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Authors:
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Erick Francisco Rakotoniriana; Gabrielle Chataigné; Guy Raoelison; Christian Rabemanantsoa; Françoise Munaut; Mondher El Jaziri; Suzanne Urveg-Ratsimamanga; Jacqueline Marchand-Brynaert; Anne-Marie Corbisier; Stéphane Declerck; Joëlle Quetin-Leclercq |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-23 |
Journal Detail:
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Title: Canadian journal of microbiology Volume: - ISSN: 1480-3275 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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a Laboratoire de microbiologie et de standardisation des médicaments, Institut malgache de recherches appliquées, B.P. 3833, Avarabohitra, Antananarivo, Madagascar. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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