Document Detail


Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic.
MedLine Citation:
PMID:  22524528     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum , Rhizoctonia solani , Colletotrichum gloeosporioides , Cryphonectria parasitica , Fusarium oxysporum , Pyrenophora tritici-repentis , Epidermophyton floccosum , and Trichophyton rubrum , was isolated from surface-sterilized Catharanthus roseus stems. Preliminary identification showed that Streptomyces cinnamoneus subsp. sparsus was its closest related species. However, strain TS3RO could readily be distinguished from this species using a combination of phenotypic properties, 16S rDNA sequence similarity, and phylogenetic analyses. Thus, the whorl-forming Streptomyces sp. strain TS3RO is likely a new subspecies within the Streptomyces cinnamoneus group. Direct bioautography on a thin-layer chromatography plate with Cladosporium cucumerinum was conducted throughout the purification steps for bioassay-guided isolation of the active antifungal compounds from the crude extract. Structural elucidation of the isolated bioactive compound was obtained via LC-MS spectrometry, UV-visible spectra, and nuclear magnetic resonance data. It revealed that fungichromin, a known methylpentaene macrolide antibiotic, was the main antifungal component of TS3RO strain, as shown by thin-layer chromatography bioautography. This is the first report of an endophytic whorl-forming Streptomyces isolated from the medically important plant Catharanthus roseus.
Authors:
Erick Francisco Rakotoniriana; Gabrielle Chataigné; Guy Raoelison; Christian Rabemanantsoa; Françoise Munaut; Mondher El Jaziri; Suzanne Urveg-Ratsimamanga; Jacqueline Marchand-Brynaert; Anne-Marie Corbisier; Stéphane Declerck; Joëlle Quetin-Leclercq
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-23
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  -     ISSN:  1480-3275     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
a Laboratoire de microbiologie et de standardisation des médicaments, Institut malgache de recherches appliquées, B.P. 3833, Avarabohitra, Antananarivo, Madagascar.
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