Document Detail

Characterization of an endogenous inhibitor of lung 15-hydroxyprostaglandin dehydrogenase.
MedLine Citation:
PMID:  3768396     Owner:  NLM     Status:  MEDLINE    
An endogenous inhibitor of the NAD+-dependent 15-hydroxyprostaglandin dehydrogenase was isolated from the 105,000 X g supernatant fraction of lungs of pregnant rabbits following DEAE chromatography. The material was heat stable and was resistant to pronase treatment. The inhibitor contained a mixture of saturated and mono-unsaturated fatty acids and cholesterol with palmitate and oleate representing the major fatty acids in the inhibitory factor. The factor inhibited prostaglandin dehydrogenase activity but had only minor effects on the activity of NAD+-dependent alcohol and lactate dehydrogenases or the NADP+-dependent isocitrate dehydrogenase. In an attempt to develop a greater understanding of the inhibitory action of fatty acids on prostaglandin dehydrogenase activity, a variety of standard fatty acids were examined for their ability to decrease enzymic activity. Oleate and palmitate inhibited enzymic activity by 70% at 10 microM, whereas arachidonate and myristate were only 30% inhibitory at this concentration. A comparison among the 18-carbon-containing fatty acids demonstrated that oleate was more potent than linoleate and linolenate in inhibiting prostaglandin dehydrogenase activity. The coenzyme A derivatives of oleate, linoleate and linolenate were less inhibitory than the free fatty acids.
J M Bergholte; R T Okita
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  879     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1986-12-09     Completed Date:  1986-12-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  164-70     Citation Subset:  IM    
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MeSH Terms
Cholesterol / analysis
Chromatography, DEAE-Cellulose
Fatty Acids / analysis
Hydroxyprostaglandin Dehydrogenases / antagonists & inhibitors*
Lung / enzymology*
NAD / metabolism
NADP / metabolism
Grant Support
Reg. No./Substance:
0/Fatty Acids; 53-59-8/NADP; 53-84-9/NAD; 57-88-5/Cholesterol; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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