| Characterization and encapsulation efficiency of rhamnolipid vesicles with cholesterol addition. | |
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MedLine Citation:
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PMID: 21489867 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The effect of cholesterol on the vesicle formation of a rhamnolipid biosurfactant extracted from the liquid culture of Pseudomonas aeruginosa SP4 was investigated. The rhamnolipid vesicles were prepared in a phosphate-buffer saline (PBS) solution (pH 7.4) at a biosurfactant concentration of 2.6mM, or 6.5 times the critical micelle concentration (CMC), with various amounts of cholesterol. The biosurfactant solution was characterized using turbidity, zeta potential, and dynamic light scattering (DLS) measurements. The morphology of the rhamnolipid vesicles formed at different cholesterol concentrations was examined with the use of transmission electron microscopy (TEM). The results showed that the rhamnolipid biosurfactant formed spherical vesicles both in the absence and presence of cholesterol, but the incorporation of the cholesterol into the bilayer membrane reduced the vesicle size. Sudan III, a water-insoluble dye, was used as a model hydrophobic compound in the encapsulation experiment. The encapsulation efficiency (E%) of the rhamnolipid vesicles was affected by the cholesterol concentration and the initial Sudan III concentration. The maximum E% of nearly 90% was achieved at the cholesterol concentration of 100μM and the initial Sudan III concentration of 8.8μM. |
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Authors:
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Orathai Pornsunthorntawee; Sumaeth Chavadej; Ratana Rujiravanit |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-11 |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: - ISSN: 1347-4421 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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