Document Detail


Characterization of embryonic stem cell lines derived from New Zealand white rabbit embryos.
MedLine Citation:
PMID:  19220131     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purposes of this study were to examine technical details in deriving and maintaining rabbit embryonic stem (rES) cell lines and to analyze their characteristics. When STO cells were used as feeder cells, no rES cell lines were established using either intact blastocysts or inner cell masses (ICMs). On the mouse embryonic fibroblasts (MEF) feeder, rES cell lines were efficiently (24%) derived. Addition of leukemia inhibitory factor (LIF) to the cells cultured on the MEF feeders further increased the derivation efficiency (57%) of rES cells. The fact that LIF induced serine-phosphorylation of STAT3 suggested LIF-dependent maintenance of rES cells. Most of the rES cell lines expressed AP, SSEA-4, Oct4, TRA-1-60, and TRA-1-81. Western blot or RT-PCR analysis also confirmed the expression of Oct4, Nanog, and Sox2. When induced to form EBs in vitro or injected to the severe combined immunodeficiency (SCID) mice, the rES cells generated embryoid bodies (EBs) and teratomas with three germ layers expressing the marker genes including MAP2, Desmin, and GATA4, respectively. In conclusion, rabbit ES cell lines can be efficiently established using our current protocols with LIF supplement. These ES cells express pluripotent stem cell markers and retain their capability to differentiate into different tissue cells. Furthermore, rES cells depend on LIF for self-renewal, likely via the JAK-STAT pathway.
Authors:
Payungsuk Intawicha; Yao-Wen Ou; Neng-Wen Lo; Su-Chun Zhang; Yin-Zhi Chen; Tzu-An Lin; Hong-Lin Su; Hwa-Fen Guu; Ming-Jer Chen; Kun-Hsiung Lee; Yung-Tsung Chiu; Jyh-Cherng Ju
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  11     ISSN:  1557-7457     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-05-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  27-38     Citation Subset:  IM    
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques*
Cell Line
Desmin / metabolism
Embryo, Mammalian / cytology*
Embryonic Stem Cells / cytology,  drug effects,  physiology*
Fibroblasts / metabolism
GATA4 Transcription Factor / metabolism
Homeodomain Proteins / metabolism
Leukemia Inhibitory Factor / pharmacology
Mice
Mice, SCID
Microtubule-Associated Proteins / metabolism
Octamer Transcription Factor-3 / metabolism
Proteoglycans / metabolism
Rabbits
SOXB1 Transcription Factors / metabolism
Stage-Specific Embryonic Antigens / metabolism
Teratoma / metabolism*
Chemical
Reg. No./Substance:
0/Desmin; 0/GATA4 Transcription Factor; 0/Homeodomain Proteins; 0/Leukemia Inhibitory Factor; 0/Microtubule-Associated Proteins; 0/Octamer Transcription Factor-3; 0/Proteoglycans; 0/SOXB1 Transcription Factors; 0/Stage-Specific Embryonic Antigens; 0/stage-specific embryonic antigen-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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