Document Detail


Characterization of the effect of high molecular weight hyaluronan on trans-synovial flow in rabbit knees.
MedLine Citation:
PMID:  9831732     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The effect of a rooster comb hyaluronan (3.6-4.0 g l-1) of similar chain length to rabbit synovial fluid hyaluronan, on the trans-synovial escape of fluid from the joint cavity in the steady state ( 8d s) was studied in 29 rabbit knees at controlled intra-articular pressures (Pj). 2. Rooster hyaluronan caused the pressure-flow relation to flatten out as pressure was raised. At 10-20 cmH2O the slope of the quasi-plateau, 0.05 +/- 0.01 microliter min-1 cmH2O-1 (mean +/- s.e.m.), was 1/39th that for Ringer solution (1.94 +/- 0.01 microliter 2O-1 ). 3. Bovine synovial fluid had a similar effect to hyaluronan in Ringer solution. 4. The quasi-plateau was caused by increasing opposition to outflow; the pressure required to drive unit outflow increased 4.4-fold between 5 and 20 cmH2O. The increased opposition to outflow at 20 cmH2O was equivalent to an effective osmotic pressure of 13-17 cmH2O at the interface. Since the infusate's osmotic pressure was only 0.9 cmH2O, this implied concentration polarization to 15-18 g l-1 hyaluronan at the interface. 5. Mechanical perforation of the lining, or enzymatic degradation of the interstitial matrix by chymopapain, abolished the quasi-plateau. Hydrational expansion of the matrix by approximately 2-fold did not. The increased opposition to outflow was reversible by washing out the hyaluronan, or by reducing Pj. It was unaffected by interruption of tissue blood flow or synoviocyte oxidative metabolism. These properties are compatible with a concentration polarization mechanism, i.e. flow-induced concentration of hyaluronan at the synovial interface due to molecular reflection. 6. A concentration polarization theory was developed for a partially reflected solute. Numerical solutions supported the feasibility of this osmotic explanation of the quasi-plateau. Additional mechanisms may also be involved. 7. It is concluded that native-size hyaluronan helps to retain synovial fluid in the joint cavity when pressure is raised and acts, at least in part, by exerting osmotic pressure at the interface between synovial matrix and a concentration polarization layer.
Authors:
P J Coleman; D Scott; R M Mason; J R Levick
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of physiology     Volume:  514 ( Pt 1)     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-11     Completed Date:  1999-03-11     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  265-82     Citation Subset:  IM    
Affiliation:
Department of Physiology, St George's Hospital Medical School, London SW17 0RE,, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Chickens
Chromatography, High Pressure Liquid
Endotoxins / pharmacology
Hyaluronic Acid / chemistry*,  isolation & purification,  pharmacology*
Isotonic Solutions / pharmacology
Knee Joint / blood supply,  metabolism*
Microcirculation / physiology
Molecular Weight
Oils
Osmotic Pressure / drug effects
Protein Structure, Tertiary
Rabbits
Synovial Fluid / drug effects,  metabolism*
Synovial Membrane / cytology,  metabolism
Time Factors
Viscosity
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Isotonic Solutions; 0/Oils; 8026-10-6/Ringer's solution; 9004-61-9/Hyaluronic Acid
Comments/Corrections

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