| Characterization of distinct apoptotic changes induced by okadaic acid and yessotoxin in the BE(2)-M17 neuroblastoma cell line. | |
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MedLine Citation:
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PMID: 11812636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptotic changes induced by okadaic acid and yessotoxin in BE(2)-M17 neuroblastoma cells have been evaluated and quantified by combining classical methods and fast and sensitive fluorimetric microplate assays. The phosphatase inhibitor okadaic acid induced rapid time- and dose-dependent apoptotic changes in this cell line, which were evident after 1h at concentrations equal or higher than 500 nM. Decreased mitochondrial membrane potential by okadaic acid (IC(50)=350 nM at 1h) was followed by cell detachment (IC(50)=400 nM at 1h), changes in total nucleic acids content (50% of controls after 1h with 1000 nM okadaic acid), caspase-3 activation (3- to 4-fold increase at 6h) and increased Annexin-V binding (1.5-fold at 6h). Yessotoxin induced similar changes in BE(2)-M17 cells, although significant differences were found in the time-course and degree of apoptotic events induced by this phycotoxin, indicating a lower potency for yessotoxin when compared with okadaic acid. This is the first report on apoptogenic activity of yessotoxin. |
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Authors:
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F Leira; C Alvarez; J M Vieites; M R Vieytes; L M Botana |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 16 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-01-28 Completed Date: 2002-03-27 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 23-31 Citation Subset: IM |
Affiliation:
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ANFACO-CECOPESCA, Campus Universitario de Vigo, 36310 Vigo, Spain. fleira@anfaco.cesga.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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metabolism Apoptosis / drug effects* Caspase 3 Caspases / biosynthesis Cell Adhesion / drug effects DNA Fragmentation / drug effects DNA, Neoplasm / analysis, biosynthesis Dose-Response Relationship, Drug Enzyme Inhibitors / toxicity* Ethers, Cyclic / toxicity* Intracellular Membranes / drug effects, physiology Membrane Potentials / drug effects, physiology Mitochondria / drug effects, physiology Mollusk Venoms / toxicity* Neuroblastoma / pathology Okadaic Acid / toxicity* Oxocins* RNA, Neoplasm / analysis, biosynthesis Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/DNA, Neoplasm; 0/Enzyme Inhibitors; 0/Ethers, Cyclic; 0/Mollusk Venoms; 0/Oxocins; 0/RNA, Neoplasm; 112514-54-2/yessotoxin; 78111-17-8/Okadaic Acid; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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