Document Detail

Characterization of distinct apoptotic changes induced by okadaic acid and yessotoxin in the BE(2)-M17 neuroblastoma cell line.
MedLine Citation:
PMID:  11812636     Owner:  NLM     Status:  MEDLINE    
Apoptotic changes induced by okadaic acid and yessotoxin in BE(2)-M17 neuroblastoma cells have been evaluated and quantified by combining classical methods and fast and sensitive fluorimetric microplate assays. The phosphatase inhibitor okadaic acid induced rapid time- and dose-dependent apoptotic changes in this cell line, which were evident after 1h at concentrations equal or higher than 500 nM. Decreased mitochondrial membrane potential by okadaic acid (IC(50)=350 nM at 1h) was followed by cell detachment (IC(50)=400 nM at 1h), changes in total nucleic acids content (50% of controls after 1h with 1000 nM okadaic acid), caspase-3 activation (3- to 4-fold increase at 6h) and increased Annexin-V binding (1.5-fold at 6h). Yessotoxin induced similar changes in BE(2)-M17 cells, although significant differences were found in the time-course and degree of apoptotic events induced by this phycotoxin, indicating a lower potency for yessotoxin when compared with okadaic acid. This is the first report on apoptogenic activity of yessotoxin.
F Leira; C Alvarez; J M Vieites; M R Vieytes; L M Botana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  16     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-28     Completed Date:  2002-03-27     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  23-31     Citation Subset:  IM    
ANFACO-CECOPESCA, Campus Universitario de Vigo, 36310 Vigo, Spain.
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MeSH Terms
Annexin A5 / metabolism
Apoptosis / drug effects*
Caspase 3
Caspases / biosynthesis
Cell Adhesion / drug effects
DNA Fragmentation / drug effects
DNA, Neoplasm / analysis,  biosynthesis
Dose-Response Relationship, Drug
Enzyme Inhibitors / toxicity*
Ethers, Cyclic / toxicity*
Intracellular Membranes / drug effects,  physiology
Membrane Potentials / drug effects,  physiology
Mitochondria / drug effects,  physiology
Mollusk Venoms / toxicity*
Neuroblastoma / pathology
Okadaic Acid / toxicity*
RNA, Neoplasm / analysis,  biosynthesis
Tumor Cells, Cultured
Reg. No./Substance:
0/Annexin A5; 0/DNA, Neoplasm; 0/Enzyme Inhibitors; 0/Ethers, Cyclic; 0/Mollusk Venoms; 0/Oxocins; 0/RNA, Neoplasm; 112514-54-2/yessotoxin; 78111-17-8/Okadaic Acid; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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