| Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector. | |
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MedLine Citation:
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PMID: 22369846 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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High-performance liquid chromatography-size exclusion chromatography (HPLC-SEC) coupled with a multiple wavelength absorbance detector (200-445nm) was used in this study to investigate the apparent molecular weight (AMW) distributions of dissolved organic matter (DOM). Standard DOM, namely humic acid, fulvic acid and hydrophilic acid, from the Suwannee River were tested to ascertain the performance and sensitivity of the method. In addition to four compounds groups: humic substances (Peak 1, AMW 16kD), fulvic acids (Peak 2, AMW 11kD), low AMW acids (Peak 3, AMW 5kD), and low AMW neutral and amphiphilic molecules, proteins and their amino acid building blocks (Peak 4, AMW 3kD), an new group that appears to include low AMW, 6-10kD, humic substances was found based on investigating the spectra at various elution times. The spectroscopic parameter S(>365) (slope at wavelengths >365nm) was determined to be a good predictor of the AMW of the DOM. The detector wavelength played an important role in evaluating the AMW distribution. For some fractions, such as the humic and low AMW non-aromatic substances, the error in measurement was ±30% as determined by two-dimensional chromatograms detected at an artificially selected wavelength. HPLC-SEC with multiple wavelength absorbance detection was found to be a useful technique for DOM characterization. It characterized the AMW distributions of DOM more accurately and provided additional, potentially important information concerning the properties of DOM with varying AMWs. |
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Authors:
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Mingquan Yan; Gregory Korshin; Dongsheng Wang; Zhenxiao Cai |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-25 |
Journal Detail:
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Title: Chemosphere Volume: - ISSN: 1879-1298 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0320657 Medline TA: Chemosphere Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, Beijing 100871, China; Department of Civil and Environmental Engineering, University of Washington, Box 352700, Seattle, WA 98195-2700, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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