Document Detail

Characterization of developmental autolysis in myxobacterial fruiting body morphogenesis with profiling of amino acids using capillary electrophoresis method.
MedLine Citation:
PMID:  15711868     Owner:  NLM     Status:  MEDLINE    
Capillary electrophoresis equipped with Laser-induced fluorescence (CE-LIF), combining with micro-culture technique was employed to determine extracellular amino acids in single myxobacterial fruiting body morphogenesis. The result showed that in the early aggregation stage, there was a remarkable increase of extracellular amino acids, which was produced by developmentally induced autolysis. The amino acids were then consumed by the vegetative cells in aggregation stage. In the following developmental events, the extracellular amino acids were kept at low level, which indicated that in the stages of fruiting body formation and myxospore development, there was no further cell autolysis. Using this novel method may provide detailed insight into the mechanisms of the developmental phenomena.
H Zhang; H Dong; J Zhao; W Hu; Y-Z Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-02-17
Journal Detail:
Title:  Amino acids     Volume:  28     ISSN:  0939-4451     ISO Abbreviation:  Amino Acids     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-13     Completed Date:  2006-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  319-25     Citation Subset:  IM    
State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
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MeSH Terms
Amino Acids / analysis,  metabolism*
Electrophoresis, Capillary / methods
Myxococcus / chemistry,  growth & development*
Spores, Bacterial / chemistry,  metabolism
Reg. No./Substance:
0/Amino Acids

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