Document Detail


Characterization of a calcium/calmodulin-dependent protein kinase homolog from maize roots showing light-regulated gravitropism.
MedLine Citation:
PMID:  8680305     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Roots of many species respond to gravity (gravitropism) and grow downward only if illuminated. This light-regulated root gravitropism is phytochrome-dependent, mediated by calcium, and inhibited by KN-93, a specific inhibitor of calcium/calmodulin-dependent protein kinase II (CaMK II). A cDNA encoding MCK1, a maize homolog of mammalian CaMK, has been isolated from roots of maize (Zea mays L.). The MCK1 gene is expressed in root tips, the site of perception for both light and gravity. Using the [35S]CaM gel-overlay assay we showed that calmodulin-binding activity of the MCK1 is abolished by 50 microM KN-93, but binding is not affected by 5 microM KN-93, paralleling physiological findings that light-regulated root gravitropism is inhibited by 50 microM KN-93, but not by 5 microM KN-93. KN-93 inhibits light-regulated gravitropism by interrupting transduction of the light signal, not light perception, suggesting that MCK1 may play a role in transducing light. This is the first report suggesting a physiological function for a CaMK homolog in light signal transduction.
Authors:
Y T Lu; H Hidaka; L J Feldman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Planta     Volume:  199     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  1996  
Date Detail:
Created Date:  1996-08-22     Completed Date:  1996-08-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  B; S    
Affiliation:
Department of Plant Biology, University of California, Berkeley 94720, USA. yingtang@nature.berkeley.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S82324
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Benzylamines / pharmacology
Calmodulin / metabolism
Cloning, Molecular
DNA, Plant
Enzyme Inhibitors / pharmacology
Gene Expression
Glycogen Synthase Kinase 3
Light
Molecular Sequence Data
Plant Roots / enzymology,  growth & development
Protein-Tyrosine Kinases / antagonists & inhibitors,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins*
Sequence Homology, Amino Acid
Sulfonamides / pharmacology
Zea mays / enzymology*
Chemical
Reg. No./Substance:
0/Benzylamines; 0/Calmodulin; 0/DNA, Plant; 0/Enzyme Inhibitors; 0/Saccharomyces cerevisiae Proteins; 0/Sulfonamides; 139298-40-1/KN 93; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.11.1/MCK1 protein, S cerevisiae; EC 2.7.11.26/Glycogen Synthase Kinase 3
Investigator
Investigator/Affiliation:
L J Feldman / U CA, Berkeley

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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