Document Detail


Characterization of delta-sleep-inducing peptide-evoked release of Met-enkephalin from brain synaptosomes in rats.
MedLine Citation:
PMID:  1861139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Delta-sleep-inducing peptide (DSIP) stimulates the release of Met-enkephalin (Met-ENK) from superfused slices of the rodent lower brainstem in vitro. In our present study, DSIP (10(-10)-10(-9) M) induced a significant release of Met-ENK from medullary synaptosomes of rats. This DSIP-evoked release of Met-ENK was Ca2+ dependent and tetrodotoxin (TTX) insensitive. Furthermore, DSIP (10(-11)-10(-9) M) significantly increased 45Ca2+ uptake in medullary synaptosomes. These results demonstrate that DSIP acts directly on the nerve endings of Met-ENK-containing neurons to release this pentapeptide by generating a Ca2+ influx into these neurons. Effects of DSIP on Met-ENK release in other discrete brain regions were also studied. Significant DSIP-evoked Met-ENK release from synaptosomes was observed in the cortex, hypothalamus, and midbrain (at concentrations of 10(-10) and 10(-9) M) and in the hippocampus and thalamus (only at 10(-9) M), but not in the striatum. In the hypothalamus, the release of Leu-enkephalin from its synaptosomes was slightly, but not significantly, enhanced by DSIP (10(-10)-10(-8) M). Our findings demonstrate that DSIP triggered a Ca2+ influx in nerve endings to induce a subsequent release of Met-ENK from neurons in only certain brain regions.
Authors:
A Nakamura; K Sakai; Y Takahashi; H Shiomi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  57     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-09-04     Completed Date:  1991-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1013-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism,  ultrastructure*
Calcium / pharmacokinetics,  physiology
Calcium Radioisotopes / diagnostic use
Cerebral Cortex / metabolism,  ultrastructure
Delta Sleep-Inducing Peptide / pharmacology*
Dose-Response Relationship, Drug
Enkephalin, Leucine / metabolism
Enkephalin, Methionine / metabolism*
Hypothalamus / metabolism,  ultrastructure
Male
Mesencephalon / metabolism,  ultrastructure
Radioimmunoassay
Rats
Rats, Inbred Strains
Synaptosomes / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Calcium Radioisotopes; 58569-55-4/Enkephalin, Methionine; 58822-25-6/Enkephalin, Leucine; 69431-45-4/Delta Sleep-Inducing Peptide; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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