Document Detail


Characterization and cytotoxicity of L-amino acid oxidase from the venom of king cobra (Ophiophagus hannah).
MedLine Citation:
PMID:  9304806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this project was to determine the cytotoxic components from the venom of king cobra, Ophiophagus hannah. Venom was purified by a combination of gel-filtration, ion-exchange and reversed-phase chromatographic steps. The biochemical properties of the cytotoxic component were consistent with those of L-amino acid oxidase. The molecular weight of the enzyme was estimated to be 150,000 by gel filtration and 70,000 under the denaturing conditions of SDS-PAGE, indicating a dimer. It has an isoelectric point of 4.5 and is a glycoprotein. The N-terminal sequence of L-amino acid oxidase from the king cobra venom was determined to be SVINLEESFQEPEYE. The cytotoxicity of L-amino acid oxidase was observed in stomach cancer, murine melanoma, fibrosarcoma, colorectal cancer and Chinese hamster ovary cell lines. Cytotoxicity resulted in the loss of ability in attachment and inhibition of cell proliferation. The cytotoxic protein decreased the level of cell proliferation by 74% according to [3H]thymidine uptake assay. The mechanism of enzyme action may be related to the inhibition of thymidine incorporation and an interaction with DNA.
Authors:
M Y Ahn; B M Lee; Y S Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The international journal of biochemistry & cell biology     Volume:  29     ISSN:  1357-2725     ISO Abbreviation:  Int. J. Biochem. Cell Biol.     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-11-17     Completed Date:  1997-11-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9508482     Medline TA:  Int J Biochem Cell Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  911-9     Citation Subset:  IM    
Affiliation:
Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Suwon, Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Oxidoreductases / chemistry*,  isolation & purification,  toxicity
Amino Acid Sequence
Amino Acids / analysis
Animals
CHO Cells
Cell Survival / drug effects
Cobra Venoms / enzymology*
Cricetinae
DNA-Binding Proteins / metabolism
Humans
L-Amino Acid Oxidase
Molecular Sequence Data
Sequence Homology, Amino Acid
Thymidine / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cobra Venoms; 0/DNA-Binding Proteins; 50-89-5/Thymidine; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.2/L-Amino Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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