Document Detail


Characterization of cytotoxic factors of Yersinia pseudotuberculosis using the MDBK cell line.
MedLine Citation:
PMID:  9853366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytotoxin of four strains of Yersinia pseudotuberculosis was characterized using the MDBK cell line and by application of the MTT colorimetric test. The highest cytotoxin yield was obtained in tryptic soy broth medium after 24 h. It was detected in the cell-free culture filtrate, and treatment of the cells with CHAPS as a membrane detergent did not decrease significantly their cytotoxic activity. The cytotoxin was inhibited by trypsinization and by increasing values of either acidity or alkalinity. The cytotoxin was inactivated partially by heating at 70 degrees C for 20 min and totally at 90 degrees C for 10 min. The results obtained indicate that the cytotoxin is protein in nature and produced mainly as free exotoxin.
Authors:
S N el-Sukhon; N Abu-Harfeil
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cytobios     Volume:  94     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1998  
Date Detail:
Created Date:  1999-02-26     Completed Date:  1999-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  15-23     Citation Subset:  IM    
Affiliation:
Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / isolation & purification,  pharmacology
Bacterial Toxins / chemistry,  isolation & purification*,  pharmacology
Cattle
Cell Line
Cholic Acids / pharmacology
Colorimetry
Kidney
Temperature
Virulence
Yersinia pseudotuberculosis / physiology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/Cholic Acids; 75621-03-3/3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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