| Characterization of cytochrome P2-450 (20-S) mRNA. Association with the P1-450 genomic gene and differential response to the inducers 3-methylcholanthrene and isosafrole. | |
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MedLine Citation:
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PMID: 6861755 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mouse liver cytochrome P2-450 is defined as the major isosafrole-inducible form of P-450 which is most specific for isosafrole metabolism. lambda AhP-1 represents a 15.5 X 10(3)-base-pair segment of mouse genomic DNA having the cytochrome P1-450 gene (approximately equal to 4600 base pairs) located in the middle portion. Using various subclones as probes, we investigated the differential expression of P1-450 mRNA and P2-450 mRNA induction as a function of association with the Ah locus, 3-methylcholanthrene or isosafrole dosage, tissue specificity, and developmental age. Both P1-450 (23-S) mRNA and P2-450 (20-S) mRNA induction processes are regulated by the Ah receptor. P2-450 mRNA is about 10-fold more sensitive than P1-450 mRNA to induction by either 3-methylcholanthrene or isosafrole. Phenobarbital pretreatment has no effect at all on either P1-450 mRNA or P2-450 mRNA. Whereas both P1-450 mRNA and P2-450 mRNA are induced by 3-methylcholanthrene in C57BL/6N liver, P1-450 (23-S) mRNA but not P2-450 (20-S) mRNA is induced by 3-methylcholanthrene in C57BL/6N kidney. P1-450 mRNA induction by 3-methylcholanthrene is measurable in C57BL/6N liver at day 15 of gestation, and the expression becomes enhanced with increasing age. P2-450 mRNA induction by 3-methylcholanthrene in C57BL/6N liver appears about 7 days later during development than 3-methylcholanthrene-inducible P1-450 mRNA. Both 3-methylcholanthrene-induced P1-450 mRNA and P2-450 mRNA are detectable in DBA/2N liver; their appearance is later in development, however, and at lower concentrations than that seen with C57BL/6N liver. P1-450 (23-S) mRNA and P2-450 (20-S) mRNA appear to hybridize to a common 5' fragment of the P1-450 gene. |
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Authors:
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T Ikeda; M Altieri; Y T Chen; M Nakamura; R H Tukey; D W Nebert; M Negishi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 134 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-08-26 Completed Date: 1983-08-26 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 13-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cloning, Molecular Cytochrome P-450 Enzyme System / biosynthesis, genetics* Dioxoles / pharmacology* Enzyme Induction / drug effects Female Gene Expression Regulation* / drug effects Kidney / enzymology Liver / enzymology Male Methylcholanthrene / pharmacology* Mice Nucleic Acid Hybridization Phenobarbital / pharmacology RNA, Messenger / isolation & purification* Safrole / pharmacology* Tetrachlorodibenzodioxin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Dioxoles; 0/RNA, Messenger; 120-58-1/isosafrole; 1746-01-6/Tetrachlorodibenzodioxin; 50-06-6/Phenobarbital; 56-49-5/Methylcholanthrene; 9035-51-2/Cytochrome P-450 Enzyme System; 94-59-7/Safrole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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