Document Detail


Characterization of cultured epithelial cells using a novel technique not requiring enzymatic digestion for subculturing.
MedLine Citation:
PMID:  23149549     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Our laboratory had developed a methodology to expand epithelial cells in culture by growing keratinocyte monolayers, under large volumes of medium that produces large numbers of keratinocytes that leave the monolayer and move into suspension. The cells have been defined as epithelial Pop Up Keratinocytes or ePUKs cells and appear to be highly suitable for clinical applications. In this publication we extend the characterization of the cells with a detailed analysis of the capabilities of the monolayer of a single culture flask to produce, over time, ePUK cells. The cells were characterized using standard epithelial markers for proliferation and differentiation. Analysis of morphology of the monolayer formed and total number of cells produced is presented for a variety of human epithelial cell strains. These keratinocytes provide an additional controlled human cell system for investigation of the mechanisms regulating epithelia cell growth and differentiation and since they are produced in large numbers, they are highly suitable for use in epithelial cell banking.
Authors:
Antonio Peramo; Stephen E Feinberg; Cynthia L Marcelo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-13
Journal Detail:
Title:  Cell and tissue banking     Volume:  -     ISSN:  1573-6814     ISO Abbreviation:  Cell Tissue Bank     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965121     Medline TA:  Cell Tissue Bank     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Surgery, University of Michigan, Ann Arbor, MI, 48109, USA, aperamo@hushmail.com.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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