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Characterization of culturable bacteria isolated from the cold-water coral Lophelia pertusa.
MedLine Citation:
PMID:  21507025     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microbes associated with corals are hypothesized to contribute to the function of the host animal by cycling nutrients, breaking down carbon sources, fixing nitrogen, and producing antibiotics. This is the first study to culture and characterize bacteria from Lophelia pertusa, a cold-water coral found in the deep sea, in an effort to understand the roles the microbes play in the coral microbial community. Two sites in the northern Gulf of Mexico were sampled over two years. Bacteria were cultured from coral tissue, skeleton, and mucus, identified by 16S rRNA genes, and subjected to biochemical testing. Most isolates were members of the Gammaproteobacteria, although there was one isolate each from the Betaproteobacteria and Actinobacteria. Phylogenetic results showed that both sampling sites shared closely related isolates (e.g., Pseudoalteromonas spp.), indicating possible temporally and geographically stable bacterial-coral associations. The Kirby-Bauer Antibiotic Susceptibility Test was used to separate bacteria to strain level, with results showing that isolates that were phylogenetically tightly grouped had varying responses to antibiotics. These results support the conclusion that phylogenetic placement cannot predict strain-level differences and further highlight the need for culture-based experiments to supplement culture-independent studies.
Authors:
J P Galkiewicz; Z A Pratte; M A Gray; C A Kellogg
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-20
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  -     ISSN:  1574-6941     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation:
College of Marine Science, University of South Florida, St. Petersburg, FL, 33701 Eckerd College, St. Petersburg, FL, 33711 U.S. Geological Survey, St. Petersburg, FL, 33701.
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