Document Detail


Characterization of cryobiological responses in TF-1 cells using interrupted freezing procedures.
MedLine Citation:
PMID:  19766619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryopreservation currently is the only method for long-term preservation of cellular viability and function for uses in cellular therapies. Characterizing the cryobiological response of a cell type is essential in the approach to designing and optimizing cryopreservation protocols. For cells used in therapies, there is significant interest in designing cryopreservation protocols that do not rely on dimethyl sulfoxide (Me(2)SO) as a cryoprotectant, since this cryoprotectant has been shown to have adverse effects on hematopoietic stem cell (HSC) transplant patients. This study characterized the cryobiological responses of the human erythroleukemic stem cell line TF-1, as a model for HSC. We measured the osmotic parameters of TF-1 cells, including the osmotically-inactive fraction, temperature-dependent membrane hydraulic conductivity and the membrane permeability to 1M Me(2)SO. A two-step freezing procedure (interrupted rapid cooling with hold time) and a graded freezing procedure (interrupted slow cooling without hold time) were used to characterize TF-1 cell recovery during various phases of the cooling process. One outcome of these experiments was high recovery of TF-1 cells cryopreserved in the absence of traditional cryoprotectants. The results of this study of the cryobiology of TF-1 cells will be critical for future understanding of the cryobiology of HSC, and to the design of cryopreservation protocols with specific design criteria for applications in cellular therapies.
Authors:
Lisa U Ross-Rodriguez; Janet A W Elliott; Locksley E McGann
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-18
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-08     Completed Date:  2010-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-16     Citation Subset:  IM    
Copyright Information:
Crown Copyright 2009. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Membrane Permeability
Cell Size
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents / pharmacokinetics
Dimethyl Sulfoxide / pharmacokinetics
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells* / cytology,  metabolism
Humans
Models, Biological
Osmosis
Grant Support
ID/Acronym/Agency:
CPG 75237//Canadian Institutes of Health Research; MOP 85068//Canadian Institutes of Health Research; MOP 86492//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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