| Characterization of crustacean hyperglycemic hormone from the crayfish (Procambarus clarkii): multiplicity of molecular forms by stereoinversion and diverse functions. | |
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MedLine Citation:
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PMID: 7821776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The complete amino acid sequence of two forms of crustacean hyperglycemic hormone (CHH) from the X-organ sinus gland complex of crayfish (Procambarus clarkii) has been determined. There are two variants of P. clarkii CHH (Prc-CHH), I and II, which can be separated by reversed-phase high-performance liquid chromatography (RP-HPLC). Each variant was oxidized by performic acid and then cleaved with lysyl endopeptidase. Intact hormone was also digested with trypsin and endoproteinase Asp-N, successively. The resulting fragments were separated by RP-HPLC and subjected to sequence analyses by a gas-phase sequencer and tandem mass spectrometry. Both variants contain 72 amino acid residues with three disulfide linkages, at positions 7-43, 23-39, and 26-52, and differ from each other by the D/L epimerization of phenylalanine at position 3; Prc-CHH-II contains D-amino acid. Injections of Prc-CHH-I and Prc-CHH-II at a dose of 12.5 pmol resulted in significant increase of hemolymph glucose levels in the crayfish. The hormones are also active in repressing ecdysteroid synthesis at concentrations of 250 mM (Prc-CHH-I) and 25 nM (Prc-CHH-II) in Y-organ culture. These results may indicate that the stereoinversion in the CHH molecule leads to an important alternation in hormonal functions during crustacean development. |
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Authors:
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A Yasuda; Y Yasuda; T Fujita; Y Naya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: General and comparative endocrinology Volume: 95 ISSN: 0016-6480 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1995-02-16 Completed Date: 1995-02-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 387-98 Citation Subset: IM |
Affiliation:
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Suntory Institute for Bioorganic Research, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Animals Astacoidea / metabolism* Chromatography, High Pressure Liquid Female Invertebrate Hormones / chemistry, metabolism, physiology Molecular Sequence Data Molecular Weight Nerve Tissue Proteins / chemistry*, metabolism, physiology* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Invertebrate Hormones; 0/Nerve Tissue Proteins; 0/hyperglycemic hormone, crustacean |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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