Document Detail


Characterization of connexin 30.3 and 43 in thymocytes.
MedLine Citation:
PMID:  15234537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During maturation, thymocytes interact directly and indirectly with different cell types of the thymic microenvironment. Such a cellular communication has been basically ascribed to soluble factors and surface receptors. However, little attention has been given to cellular communication mediated by gap junctions. The existence of these intercellular channels in the immune system remained a controversial issue since the 1970s until recently, when a growing body of evidence has indicated their presence and physiological roles in the immune system. In this work, we investigated whether thymocytes express gap junction-forming proteins (connexins, Cx) and are capable of forming functional intercellular channels. Using RT-PCR, we demonstrated that thymocytes express the mRNA for two Cx isoforms: Cx30.3 and Cx43, but not for Cx26, Cx30, Cx31, Cx31.1, Cx32, Cx33, Cx36, Cx37, Cx40, Cx45, Cx46, and Cx50. In addition, the presence of Cx30.3 and Cx43 was confirmed using different techniques (RNase protection assay, western blot and immunofluorescence). However, despite the expression of these two Cxs, we did not detect functional homocellular coupling between thymocytes or between EL-4 cells (a Cx43 expressing thymic lymphoma-derived cell line) or heterocellular coupling between thymocytes and thymic epithelial cells (TEC) or between EL-4 and TEC in unstimulated conditions. Concluding, in this study, we described for the first time the expression of connexins in thymocytes, which may constitute a new molecule having a functional role in thymocytes maturation.
Authors:
Paula Candida Fonseca; Oscar Kenji Nihei; Márcia Urban-Maldonado; Simone Abreu; Antonio Carlos Campos de Carvalho; David C Spray; Wilson Savino; Luiz Anastacio Alves
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology letters     Volume:  94     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-05     Completed Date:  2005-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  65-75     Citation Subset:  IM    
Affiliation:
Laboratório de Pesquisas sobre o Timo, Departamento de Imunologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Brasil, Av. Brasil, 4365 Manguinhos, 21045-900, Rio de Janeiro, RJ, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology*
Cells, Cultured
Connexin 43 / analysis,  genetics,  metabolism*
Connexins / analysis,  genetics,  metabolism*
Gap Junctions / metabolism
Gene Expression
Mice
Protein Isoforms / genetics,  metabolism
T-Lymphocytes / immunology,  physiology*
Thymus Gland / cytology*,  immunology,  metabolism
Chemical
Reg. No./Substance:
0/Connexin 43; 0/Connexins; 0/Protein Isoforms; 147652-21-9/connexin 30.3
Comments/Corrections
Erratum In:
Immunol Lett. 2005 Apr 15;98(1):173-5

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