| Characterization of cis elements that regulate the expression of glnA in Synechococcus sp. strain PCC 7942. | |
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MedLine Citation:
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PMID: 7721715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The upstream noncoding region of the Synechococcus sp. strain PCC 7942 (hereafter referred to as Synechococcus 7942) glnA gene was fused to the cat gene in order to study the expression of glnA both in Synechococcus 7942 and in Escherichia coli. The lack of cat expression in E. coli indicated that the glnA promoter was not recognized by E. coli RNA polymerase. The fused construct was integrated into the Synechococcus 7942 chromosome at a neutral site. Expression of the cat reporter gene was regulated under various nitrogen conditions in a way similar to that of the glnA gene. A deletion introduced at the binding site of the NtcA regulatory protein abolished derepression of the glnA promoter during growth in nitrate and under nitrogen starvation. Deletion of the sequence between the transcription and translation start sites of glnA prevented the repression observed during growth in ammonium. These results indicate that the glnA promoter is subject to complex regulation that involves sequences upstream and downstream from the transcription start site. |
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Authors:
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R Cohen-Kupiec; A Zilberstein; M Gurevitz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 177 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-05-19 Completed Date: 1995-05-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2222-6 Citation Subset: IM |
Affiliation:
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Department of Botany, Tel Aviv University, Ramat-Aviv, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Binding Sites / genetics Cloning, Molecular Cyanobacteria / genetics*, growth & development, metabolism DNA Primers / genetics DNA, Bacterial / genetics Escherichia coli / genetics Gene Expression Regulation, Bacterial Genes, Bacterial* Genes, Reporter Molecular Sequence Data Nitrogen / metabolism Promoter Regions, Genetic Sequence Deletion |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Bacterial; 7727-37-9/Nitrogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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