| Characterization of a chronic infection in an internally-stabilized segmental defect in the rat femur. | |
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MedLine Citation:
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PMID: 16022995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to characterize a new model of chronic osteomyelitis with clinically relevant features. A segmental defect of critical size was surgically created in the rat femur, stabilized with a polyacetyl plate and Kirschner wires, and contaminated with bacteria. The animals were allowed to recover while the contamination progressed to a chronic infection. At a later point in time, the defect was surgically débrided without removing the implant. Further treatments of interest, such as antibiotic therapy or application of an osteogenic agent, could be introduced at this time. To implement this model, an initial experiment was performed to determine the bacterial inoculum and time from contamination that would reliably result in an infected defect without causing excessive bone damage by the time débridement surgery was performed. The number of recovered bacteria, degree of radiographic bony lysis, and torsional stiffness of the defect fixation were measured in 192 rats as a function of 4 inocula of Staphylococcus aureus (10(3), 10(4), 10(5) or 10(6) CFUs) and 4 times from contamination (1, 2, 3 or 4 weeks). A 10(4) CFU inoculum over 2 weeks was found to consistently create an infection without severe lysis and loss of fixation stability. Based on these values, a second experiment was performed in 96 rats to characterize the débrided defect over time (2, 4, 8 and 12 weeks after débridement), with and without 4 weeks of the antibiotic ceftriaxone, in terms of the same outcome variables. Infection was persistent in all animals in spite of débridement and antibiotic therapy. Antibiotic therapy did not reduce the degree of bony lysis. Compared with animals not given antibiotic, bacterial counts significantly decreased during the period of antibiotic therapy, but then rebounded to significantly higher levels at 12 weeks. This model allows us to perform further studies on differing regimens of antibiotic therapy and their relationship to surgical débridement, and on the efficacy of osteogenic agents in the presence of infection. |
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Authors:
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Xinqian Chen; Dean T Tsukayama; Louis S Kidder; Craig A Bourgeault; Andrew H Schmidt; William D Lew |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-04-07 |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: 23 ISSN: 0736-0266 ISO Abbreviation: J. Orthop. Res. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-18 Completed Date: 2005-08-30 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: United States |
Other Details:
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Languages: eng Pagination: 816-23 Citation Subset: IM |
Affiliation:
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Orthopaedic Biomechanics Laboratory, Midwest Orthopaedic Research Foundation and Minneapolis Medical Research Foundation, 914 South 8th Street, MC860C, Minneapolis, MN 55404, USA. pchen@mmrf.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Bacterial Agents / pharmacology* Bacteria / growth & development Chronic Disease Combined Modality Therapy Debridement Disease Models, Animal Femur / microbiology, radiography, surgery Fracture Fixation, Internal* Osteolysis / microbiology, radiography, surgery Osteomyelitis / drug therapy*, radiography, surgery* Rats Rats, Sprague-Dawley Staphylococcal Infections / drug therapy*, radiography, surgery* Torsion Abnormality |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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