| Characterization of the chondroitin sulfate produced by B16 mouse melanoma cells. | |
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MedLine Citation:
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PMID: 902275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mucopolysaccharides produced by B16 mouse melanoma cells have been isolated in milligram quantities from the spent media in which the cells were grown in the presence of 2-amino-2-deoxy-D-glucose-t and [35S]-sulfate. The mucopolysaccharides obtained by precipitation with cetylpyridinium chloride from the Pronase digest of the media were further purified by gel filtration, ion-exchange chromatography, and treatment with nucleases. The major components were identified as chondroitin-4-sulfates by identification of the hexosamine as 2-amino-2-deoxy-D-galactose, and by digestibility with hyaluronidases, chondroitinase AC, and chondro-4-sulfatase. The o.r.d. curve and i.r. spectra of these components also confirmed their similarity to chondroitin-4-sulfate from cartilage. The molecular weight of the polysaccharide chains was estimated to be in the range 90,000-120,000 by sedimentation equilibrium analysis. |
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Authors:
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V P Bhavanandan; E A Davidson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carbohydrate research Volume: 57 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1977 Aug |
Date Detail:
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Created Date: 1977-11-25 Completed Date: 1977-11-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 173-88 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrates
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analysis Cell Line Chondroitin* / analogs & derivatives Chondroitin Sulfates* / isolation & purification, metabolism Hexosamines / analysis Melanoma / metabolism Molecular Weight Optical Rotatory Dispersion Spectrophotometry, Infrared |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Hexosamines; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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