Document Detail

Characterization of the chondroitin sulfate produced by B16 mouse melanoma cells.
MedLine Citation:
PMID:  902275     Owner:  NLM     Status:  MEDLINE    
The mucopolysaccharides produced by B16 mouse melanoma cells have been isolated in milligram quantities from the spent media in which the cells were grown in the presence of 2-amino-2-deoxy-D-glucose-t and [35S]-sulfate. The mucopolysaccharides obtained by precipitation with cetylpyridinium chloride from the Pronase digest of the media were further purified by gel filtration, ion-exchange chromatography, and treatment with nucleases. The major components were identified as chondroitin-4-sulfates by identification of the hexosamine as 2-amino-2-deoxy-D-galactose, and by digestibility with hyaluronidases, chondroitinase AC, and chondro-4-sulfatase. The o.r.d. curve and i.r. spectra of these components also confirmed their similarity to chondroitin-4-sulfate from cartilage. The molecular weight of the polysaccharide chains was estimated to be in the range 90,000-120,000 by sedimentation equilibrium analysis.
V P Bhavanandan; E A Davidson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carbohydrate research     Volume:  57     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-11-25     Completed Date:  1977-11-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  173-88     Citation Subset:  IM    
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MeSH Terms
Carbohydrates / analysis
Cell Line
Chondroitin* / analogs & derivatives
Chondroitin Sulfates* / isolation & purification,  metabolism
Hexosamines / analysis
Melanoma / metabolism
Molecular Weight
Optical Rotatory Dispersion
Spectrophotometry, Infrared
Reg. No./Substance:
0/Carbohydrates; 0/Hexosamines; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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