Document Detail


Characterization of cholinesterase activity in three bivalves inhabiting the North Adriatic sea and their possible use as sentinel organisms for biosurveillance programmes.
MedLine Citation:
PMID:  12873401     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anticholinesterases constitute a major portion of modern synthetic insecticides and the assessment of cholinesterase (ChE) inhibition is widely used as a specific biomarker for evaluating the exposure of non-target organisms to these pollutants. To evaluate the possible exposure of the North Adriatic sea coastal environment to residues of agriculture practices, we ascertained whether the oyster, Ostrea edulis and the clam, Tapes philippinarum, highly valuable resources commercially harvested in the area, could be selected as sentinel species. We characterized the ChE biochemical properties in their gills, and for comparison the analysis was carried out also in gills of the mussel, Mytilus galloprovincialis, extensively used as a biological indicator of water quality. In the oysters and mussels, the ChE activity was a function of increasing concentrations of substrate in the range 0.01-0.5 mM. K(m) values were 93+/-15 and 77+/-8 microM for O. edulis and M. galloprovincialis, respectively. No detectable ChE activity was found in gills of T. philippinarum, at any tested condition. Significant inhibition of ChE activity by eserine, carbaryl, and ethyl-paraoxon was observed in gills of O. edulis and M. galloprovincialis. Although field validation is needed, the present study suggests that O. edulis may be employed as a biological indicator for assessing pesticide contamination, whilst T. philippinarum does not seem useful for this purpose.
Authors:
Paola Valbonesi; Giorgio Sartor; Elena Fabbri
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Science of the total environment     Volume:  312     ISSN:  0048-9697     ISO Abbreviation:  Sci. Total Environ.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-22     Completed Date:  2003-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0330500     Medline TA:  Sci Total Environ     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  79-88     Citation Subset:  IM    
Affiliation:
Interdepartment Centre for Environmental Science Research, University of Bologna, via Tombesi dall'Ova 55, 48100 Ravenna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis
Bivalvia / enzymology
Cholinesterases / analysis,  pharmacology*
Environmental Monitoring / methods*
Gills / enzymology
Mollusca / enzymology*
Ostreidae / enzymology
Pesticides / adverse effects*
Sensitivity and Specificity
Water Pollutants, Chemical / adverse effects*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Pesticides; 0/Water Pollutants, Chemical; EC 3.1.1.8/Cholinesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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