Document Detail


Characterization of chiral interactions using fluorescence anisotropy.
MedLine Citation:
PMID:  17165843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work details a study whereby the characterization of chiral selectors and identification of optimal separation conditions is evaluated by steady-state fluorescence anisotropy measurements. Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evaluating chiral recognition, and in this study, the feasibility of characterizing chiral separation systems by the technique is evaluated. Four chiral selectors were examined under various conditions to explore correlation between chiral separation ability and differences in the steady-state fluorescence anisotropy of the enantiomers measured under similar conditions. A good correlation between the fluorescence anisotropy data and separation data was observed with R2 values ranging from 0.9279 to 0.9959. The fluorescence anisotropy measurements were examined under conditions that mimicked chiral separation conditions and the feasibility of a priori optimization of chiral separations is discussed.
Authors:
Irene W Kimaru; Yafei Xu; Matthew E McCarroll
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-14     Completed Date:  2007-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8485-90     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois 62901, USA.
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MeSH Terms
Descriptor/Qualifier:
Fluorescence Polarization*
Microchemistry
Molecular Structure
Stereoisomerism
Sulfates / chemistry*
beta-Cyclodextrins / chemistry*
Chemical
Reg. No./Substance:
0/Sulfates; 0/beta-Cyclodextrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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