Document Detail

Characterization of the chicken small intestine type IIb sodium phosphate cotransporter.
MedLine Citation:
PMID:  17179418     Owner:  NLM     Status:  MEDLINE    
Intestinal absorption and renal resorption play a critical role in overall phosphorus homeostasis in chickens. Using RNase-ligase-mediated rapid amplification of cDNA ends PCR, we obtained a cDNA from the broiler small intestine that encodes a type IIb Na-dependent phosphate transporter. The cDNA has an open reading frame of 2,022 bp and predicts a 674-amino acid protein with a molecular mass of approximately 74 kDa. Prediction of membrane spanning domains based on the hydrophilic and hydrophobic properties of the amino acids suggests 8 transmembrane domains, with both the NH(2) and COOH termini being intracellular. The Na-inorganic phosphate (Pi) IIb cotransporter has relative high homology with other type II Na-Pi cotransporters but low homology with the type I or type III Na-Pi cotransporters. Northern blot analysis demonstrated the presence of a single mRNA transcript present predominantly in the small intestine, with the highest expression in the duodenum, followed by the jejunum and ileum. In situ hybridization indicated that the Na-Pi cotransporter mRNA is expressed throughout the vertical cryptvillus axis of the small intestine. Reduction of P in the diet of chicks from hatch to 4 d of age resulted in a significant induction of Na-Pi cotransporter mRNA expression in the small intestine. Further study is needed to elucidate its physiological role in intestinal phosphate absorption in chickens.
F Yan; R Angel; C M Ashwell
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Publication Detail:
Type:  Clinical Trial; Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  86     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-03-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
Department of Animal and Avian Sciences, University of Maryland, College Park 20742, USA.
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MeSH Terms
Amino Acid Sequence
Animal Feed
Base Sequence
Chickens / genetics,  metabolism*
Cloning, Molecular
Diet / veterinary
Gene Expression Regulation
Intestine, Small / cytology,  metabolism*
Molecular Sequence Data
Phosphorus, Dietary / metabolism,  pharmacology
Quantitative Trait Loci
Sodium-Phosphate Cotransporter Proteins, Type IIb / chemistry,  genetics,  metabolism*
Reg. No./Substance:
0/Phosphorus, Dietary; 0/Sodium-Phosphate Cotransporter Proteins, Type IIb

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