| Characterization of changes in global gene expression in the brain of neuron-specific enolase/human Tau23 transgenic mice in response to overexpression of Tau protein. | |
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MedLine Citation:
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PMID: 20372808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tau is a neuronal phosphoprotein responsible for the formation of the neurofibrillary tangles in Alzheimer's disease. To characterize the changes in global gene expression in the brain of transgenic mice that overexpress human Tau23 protein in response to the increase of Tau23 phosphorylation, total RNA extracted from the hippocampus of 12-month-old transgenic and wild-type mice was converted to cDNA, labeled with biotin and hybridized to oligonucleotide microarrays. The microarray results were confirmed by real-time RT-PCR and Western blotting method. It was determined that 43 genes were up-regulated and 8 genes were down-regulated by Tau23 in transgenic mice compared to controls, based on the arbitrary difference in the 2-fold change. Among the up-regulated transcripts, those encoding for transporter and oxidoreductase were dramatically over-represented, followed by those related to regulatory molecule, cytoskeletal protein, signaling molecule, and extracellular matrix protein. Genes encoding for transcription factor, regulatory molecule, miscellaneous function, and chaperone were significantly reduced in the down-regulated group. The major genes in the up-regulated categories included Ecrg4, Folr1, Defb11, Aqp1 and Soctdc1. The major genes in the down-regulated categories were Ncor1, Gpm6a, and Hspd1. These results indicate that the microarray analysis identifies several gene functional groups and individual genes that respond to a sustained increase in Tau23 phosphorylation levels in the brain of transgenic mice. In addition, the results suggest the microarray test is a useful tool for increased understanding of the role of Tau23 protein in regulating neurodegenerative disorders. |
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Authors:
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Jong-Min Woo; So Jung Park; Ho Il Kang; Byoung Guk Kim; Sun Bo Shim; Seung Wan Jee; Su Hae Lee; Ji Soon Sin; Chang Joon Bae; Mee Kyung Jang; Chunghee Cho; Dae Youn Hwang; Chuel Kyu Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 25 ISSN: 1791-244X ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-07-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 667-75 Citation Subset: IM |
Affiliation:
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Division of Laboratory Animal Resources, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Seoul 122-704, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / physiology* Female Gene Expression Profiling Gene Expression Regulation* Humans Male Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Transgenic* Molecular Sequence Data Oligonucleotide Array Sequence Analysis Phosphopyruvate Hydratase* / genetics, metabolism tau Proteins* / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/tau Proteins; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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