Document Detail

Characterization of cells with proliferative activity after a brain injury.
MedLine Citation:
PMID:  15737436     Owner:  NLM     Status:  MEDLINE    
The cellular responses to a brain injury are important steps in restoring the integrity and function of the brain. Proliferating cells, such as reactive astrocytes, oligodendrocyte precursor cells and microglia remodel the injured tissue. To spatially and temporally characterize the initial cellular responses in vivo, proliferating cells were pulse-labeled with BrdU soon after (the 2nd day) a cortical cryo-injury, and their fate was investigated by double labeling with an anti-BrdU antibody and antibodies to various cellular markers. Three days after the cryo-injury, a significant proportion of BrdU-positive cells were positive for NG2-proteoglycan, suggesting that oligodendrocyte progenitors (OPCs) were induced in response to injury. One-two weeks after the cryo-injury, the number of OPC was reduced and GFAP/BrdU double-positive cells, in turn, became dominant, while cells with mature oligodendrocyte markers did not increase significantly. Neuronal markers were rarely co-localized with BrdU immunoreactivity throughout the period studied. These findings imply that OPCs are prone to differentiate to astrocytes in the lesioned site. In this cryo-injury model, treatment with thyroid hormone (T4) altered cell fate; the increase in the number of GFAP/BrdU-positive cells was significantly diminished, and there was an increased number of mature oligodendrocytes (CNPase, PLP-positive) exhibiting BrdU immunoreactivity. These findings suggest that modification of proliferating progenitors in injured brain by hormonal or chemical treatment might benefit functional regeneration.
Kouko Tatsumi; Satomi Haga; Hiroko Matsuyoshi; Masahide Inoue; Takayuki Manabe; Manabu Makinodan; Akio Wanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemistry international     Volume:  46     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-01     Completed Date:  2005-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Copyright Information:
(c) 2005 Elsevier Ltd. All rights reserved.
Department of Anatomy, Nara Medical University, 840 Shijo-cho, Kashihara City, Nara 634-8521, Japan.
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MeSH Terms
2',3'-Cyclic-Nucleotide Phosphodiesterases / metabolism
Antigens / metabolism
Astrocytes / drug effects,  metabolism,  pathology
Biological Markers / metabolism
Brain / drug effects,  metabolism*,  pathology
Brain Injuries / pathology,  physiopathology*,  therapy
Cell Differentiation / drug effects,  physiology
Cell Lineage / drug effects,  physiology
Cell Proliferation / drug effects
Disease Models, Animal
Glial Fibrillary Acidic Protein / metabolism
Nerve Regeneration / drug effects,  physiology*
Neuronal Plasticity / drug effects,  physiology*
Oligodendroglia / drug effects,  metabolism,  pathology
Proteoglycans / metabolism
Stem Cells / drug effects,  metabolism*,  pathology
Thyroxine / metabolism,  pharmacology
Reg. No./Substance:
0/Antigens; 0/Biological Markers; 0/Glial Fibrillary Acidic Protein; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4; 59-14-3/Bromodeoxyuridine; 7488-70-2/Thyroxine; EC 3.1.4.-/2',3'-Cyclic-Nucleotide Phosphodiesterases

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