Document Detail

Characterization of cell proliferation in rat spinal cord following peripheral nerve injury and the relationship with neuropathic pain.
MedLine Citation:
PMID:  17560721     Owner:  NLM     Status:  MEDLINE    
Glial activation is a typical response of the central nervous system to nerve injury. In the current investigation, we characterized the temporal and spatial pattern of glial proliferation, one of the most conspicuous features of glial activation, in relation to nerve injury-induced neuropathic pain. Using bromodeoxyuridine (BrdU) as a mitotic marker, we analyzed cell proliferation in the spinal cord, identified the phenotype of dividing cells, traced their fate, and correlated these phenomena with behavioural assays of the neuropathic pain syndrome. Our results demonstrated that peripheral nerve injury induced an early and transient cell proliferation, on the spinal cord ipsilateral to the nerve lesion which peaked at day 3 post-surgery. The majority of the proliferating cells were Iba-1(+) microglia, together with some NG2(+) oligodendrocyte progenitors, and GFAP(+) astrocytes. These newly generated cells continued to divide over time with the response peaking at day 14 post-injury. Microglia were always the predominant phenotype which made up over 60% of activated microglia derived from this newly generated cell population. There was a close temporal correlation between microglial proliferation in the spinal cord dorsal horn and the abnormal pain responses, suggesting a contribution of the new microglia to the genesis of the neuropathic pain symptoms.
Stefania Echeverry; Xiang Qun Shi; Ji Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-08
Journal Detail:
Title:  Pain     Volume:  135     ISSN:  1872-6623     ISO Abbreviation:  Pain     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7508686     Medline TA:  Pain     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  37-47     Citation Subset:  IM    
Unité de Neurobiologie Cellulaire, Centre de Recherche Université Laval Robert-Giffard, Université Laval, 2601, Chemin de la Canardière, Québec, Canada.
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MeSH Terms
Analysis of Variance
Antigens, CD11b / metabolism
Bromodeoxyuridine / metabolism
Calcium-Binding Proteins / metabolism
Cell Proliferation*
Functional Laterality
Lumbosacral Region
Microglia / physiology
Nerve Tissue Proteins / metabolism
Neuralgia / etiology*,  pathology
Pain Measurement / methods
Peripheral Nervous System Diseases / complications*,  pathology*
Rats, Sprague-Dawley
Spinal Cord / pathology*
Time Factors
Reg. No./Substance:
0/Aif1 protein, rat; 0/Antigens, CD11b; 0/Calcium-Binding Proteins; 0/Nerve Tissue Proteins; 59-14-3/Bromodeoxyuridine

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