Document Detail


Characterization of cell deformation and migration using a parametric estimation of image motion.
MedLine Citation:
PMID:  10230137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper deals with the spatio-temporal analysis of two-dimensional deformation and motion of cells from time series of digitized video images. A parametric motion approach based on an affine model has been proposed for the quantitative characterization of cellular movements in different experimental areas of cellular biology including spontaneous cell deformation, cell mitosis, individual cell migration and collective migration of cell populations as cell monolayer. The accuracy and robustness of the affine model parameter estimation, which is based on a multiresolution algorithm, has been established from synthesized image sequences. A major interest of our approach is to follow with time the evolution of a few number of parameters characteristic of cellular motion and deformation. From the time-varying eigenvalues of the affine model square matrix, a precise quantification of the cell pseudopodial activity, as well as of cell division has been performed. For migrating cells, the motion quantification confirms that cell body deformation has a leading role in controlling nucleus displacement, the nucleus itself undergoing a larger rotational motion. At the cell population level, image motion analysis of in vitro wound healing experiments quantifies the heterogeneous cell populations dynamics.
Authors:
F Germain; A Doisy; X Ronot; P Tracqui
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  46     ISSN:  0018-9294     ISO Abbreviation:  IEEE Trans Biomed Eng     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-03     Completed Date:  1999-06-03     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  584-600     Citation Subset:  IM    
Affiliation:
Laboratoire TIMC, UMR CNRS 5525, Faculté de Médecine, La Tronche, France.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Cell Division / physiology
Cell Movement*
Cell Nucleus / physiology
Fibroblasts / cytology*
Image Processing, Computer-Assisted*
Linear Models*
Mice
Models, Biological*
Motion
Wound Healing / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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