| Characterization and carbon metabolism in fungi pathogenic to Triatoma infestans, a chagas disease vector. | |
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MedLine Citation:
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PMID: 11023748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathogenicity of Metarhizium anisopliae (Ma) and Beauveria bassiana (Bb) isolates against Triatoma infestans, the major vector of Chagas disease in Argentina is reported. A 100% mortality was achieved with mean lethal times varying form 5.8 (Ma6) to 7.7 (Bb5) or 11.1 days (Bb10). The fatty acid, hydrocarbon, and total lipid patterns were compared for glucose-grown and alkane-grown Bb10 cultures. The alkane-grown cells showed a lipid pattern different from that of glucose-grown cells, with triacylglyercol as the major lipid fraction, whereas sterols prevailed in the glucose-grown cells. A significant reduction in the relative amounts of linoleic acid diminished the unsaturated/saturated fatty acid ratio for alkane-grown cells; in addition, large amounts of heptacosanoic and eicosanoic acids were detected in the saturated fraction. The hydrocarbon profile of Bb10 showed a saturated chain length distribution,with a marked prevalence for straight chains, ranging from n-C18 to n-C37 in the carbon skeleton, with n-C22 as the major component. Alkane-grown cells showed no qualitative changes in their hydrocarbon fraction, but a similar ratio for odd/even carbon chains. After 48-h incubation assays,[1-(14)C]acetate uptake was largely diminished following a period of alkane growth induction. Glucose-grown cells readily incorporated 19% of the labelinto phospholipids, hydrocarbons, triacylglycerols, and free fatty acids. In contrast, incorporation was reduced to 5.3% for alkane-grown cells, accounting only for phospholipid synthesis. |
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Authors:
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M P Juárez; R Crespo; G C Fernández; R Lecuona; L F Cafferata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of invertebrate pathology Volume: 76 ISSN: 0022-2011 ISO Abbreviation: J. Invertebr. Pathol. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-11-06 Completed Date: 2000-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0014067 Medline TA: J Invertebr Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 198-207 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Academic Press. |
Affiliation:
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Facultad de Ciencias Médicas, Instituto de Investigaciones Bioquímicas de La Plata, UNLP, calles 60 y 120, La Plata, 1900, Argentina. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon / metabolism* Chagas Disease / transmission* Insect Vectors* Mitosporic Fungi / metabolism, pathogenicity, physiology* Triatoma / microbiology* |
| Chemical | |
Reg. No./Substance:
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7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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