| Characterization of the carbohydrate backbone of Vibrio parahaemolyticus O6 lipopolysaccharides. | |
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MedLine Citation:
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PMID: 21639862 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Structural characterization studies have been carried out on the carbohydrate backbone of Vibrio parahaemolyticus serotype O6 lipopolysaccharides (LPS). The carbohydrate backbone isolated from O6 LPS by sequential derivatization, i.e., dephosphorylation, O-deacylation, pyridylamination, N-deacylation and N-acetylation, is a nonasaccharide consisting of 3 mol of D-glucosamine (GlcN) (of which one is pyridylaminated), 2 mol of L-glycero-D-manno-heptose (Hep), and 1 mol each of D-galactose (Gal), D-glucose (Glc), D-glucuronic acid (GlcA) and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo). Structural analyses by NMR spectroscopy and fast-atom bombardment mass spectrometry (FABMS) demonstrated that the carbohydrate backbone is β-Galp-(1→2)-α-Hepp-(1→3)-α-Hepp-(1→5)-α-Kdop-(2→6)-β-GlcpNAc-(1→6)-GlcNAc-PA, in which the 3-substituted α-Hepp is further substituted by β-GlcpNAc-(1→4)-β-Glcp at position 4 and by β-GlcpA at position 2. In native O6 LPS, an additional 1 mol of D-galacturonic acid, which is liberated by dephosphorylation in hydrofluoric acid, is present at an unknown position. Our previous report described that of 13 O-serotype LPS of V. parahaemolyticus, the only LPS from which Kdo was detected was from O6 LPS after mild acid hydrolysis. In the present study, we demonstrate that only 1 mol of Kdo is present at the lipid A proximal position, which is common component among the LPS in all serotypes of the bacterium, and that no additional Kdo is present in the carbohydrate backbone of O6 LPS. Enzyme-linked immunosorbent assay (ELISA) and ELISA inhibition analysis using antisera against O6 and Salmonella enterica Minnesota R595 and LPS of both strains further revealed that Kdo is not involved as an antigenic determinant of O6 LPS. |
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Authors:
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Yasunori Isshiki; Seiichi Kondo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-6 |
Journal Detail:
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Title: Microbiology and immunology Volume: - ISSN: 1348-0421 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Societies and Blackwell Publishing Asia Pty Ltd. |
Affiliation:
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Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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